TY - JOUR
T1 - Retrovirus-mediated gene transfer and expression cloning
T2 - Powerful tools in functional genomics
AU - Kitamura, Toshio
AU - Koshino, Yuko
AU - Shibata, Fumi
AU - Oki, Toshihiko
AU - Nakajima, Hideaki
AU - Nosaka, Tetsuya
AU - Kumagai, Hidetoshi
N1 - Funding Information:
This work was supported by grants from the Ministry of Education, Science, Sports and Culture and the Ministry of Health and Welfare, Japan. The division of Hematopoietic Factors is supported by Chugai Pharmaceutical Co., Ltd. The authors thank Drs. Carol Stocking and Wolfram Ostertag for MPEV and FMEV vectors and valuable discussions, Drs. Hirofumi Hamada and Hideo Iba for the valuable discussions, and M. Ohara for excellent language assistance.
PY - 2003/11
Y1 - 2003/11
N2 - Most of the human genome has now been sequenced and about 30,000 potential open reading frames have been identified, indicating that we use these 30,000 genes to functionally organize our biologic activities. However, functions of many genes are still unknown despite intensive efforts using bioinformatics as well as transgenic and knockout mice. Retrovirus-mediated gene transfer is a powerful tool that can be used to understand gene functions. We have developed a variety of retrovirus vectors and efficient packaging cell lines that have facilitated the development of efficient functional expression cloning methods. In this review, we describe retrovirus-mediated strategies used for investigation of gene functions and function-based screening strategies.
AB - Most of the human genome has now been sequenced and about 30,000 potential open reading frames have been identified, indicating that we use these 30,000 genes to functionally organize our biologic activities. However, functions of many genes are still unknown despite intensive efforts using bioinformatics as well as transgenic and knockout mice. Retrovirus-mediated gene transfer is a powerful tool that can be used to understand gene functions. We have developed a variety of retrovirus vectors and efficient packaging cell lines that have facilitated the development of efficient functional expression cloning methods. In this review, we describe retrovirus-mediated strategies used for investigation of gene functions and function-based screening strategies.
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U2 - 10.1016/S0301-472X(03)00260-1
DO - 10.1016/S0301-472X(03)00260-1
M3 - Review article
C2 - 14585362
AN - SCOPUS:0142227052
SN - 0301-472X
VL - 31
SP - 1007
EP - 1014
JO - Experimental Hematology
JF - Experimental Hematology
IS - 11
ER -