TY - JOUR
T1 - Resurgence of rotavirus genotype G12 in St. Louis during the 2014-2015 rotavirus season
AU - Wylie, Kristine M.
AU - Stanley, Katherine M.
AU - TeKippe, Erin Mc Elvania
AU - Mihindukulasuriya, Kusal
AU - Storch, Gregory A.
N1 - Funding Information:
Acknowledgments. We thank Richard Buller and Stephanie Bledsoe from the Clinical Virology Laboratory at Children’s Hospital in St. Louis for providing samples and Brandi Herter and Maria Cannella for their assistance with the high-throughput sequencing and virus subtyping assays. Financial support. This work was supported by institutional funds from the Washington University Department of Pediatrics. The Virus Pathogen Database and Analysis Resource, a publicly available resource used in this study, has been wholly funded with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272200900041C. Potential conflicts of interest. All authors: No reported conflicts.
Funding Information:
We thank Richard Buller and Stephanie Bledsoe from the Clinical Virology Laboratory at Children's Hospital in St. Louis for providing samples and Brandi Herter and Maria Cannella for their assistance with the high-throughput sequencing and virus subtyping assays. This work was supported by institutional funds from the Washington University Department of Pediatrics. The Virus Pathogen Database and Analysis Resource, a publicly available resource used in this study, has been wholly funded with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272200900041C. Potential conflicts of interest. All authors: No reported conflicts.
Publisher Copyright:
© The Author 2016. Published by Oxford University Press on behalf of The Journal of the Pediatric Infectious Diseases Society. All rights reserved.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Background. Rotaviruses are a leading cause of gastroenteritis. Rotavirus vaccination has dramatically reduced rotavirus occurrence; however, we have noticed mild to moderate recurrences in the St. Louis area in alternate years. In 2013, we found rotavirus genotype G12 to be the dominant strain in the St. Louis region. In this study, we again determined the distribution of genotypes and ascertained vaccine history in patients infected with rotavirus G12 during the 2014-15 season. Methods. Samples submitted to the St. Louis Children's Hospital Microbiology Laboratory were tested for rotavirus using an antigen assay. We determined the VP7 genotype using amplicon sequence analysis. We determined genome sequences using high-throughput sequencing. We evaluated rotavirus immunization records when available. Results. Of 30 typed viruses from 2014-15, 29 were G12 (97%). Whole-genome sequencing revealed few changes from G12 viruses analyzed in 2012-13. VP4 and VP7 sequences were > 99% identical to previously sequenced G12 strains from St. Louis, and immune epitopes were conserved. Vaccination histories were available from 17 patients. Of these, 4 had been vaccinated, 3 had received incomplete vaccination or had a vaccine history that could not be confirmed, and 10 had not been vaccinated. Conclusions. G12 re-emerged as the predominant rotavirus genotype in 2014-15, comprising a higher percentage of cases than in 2012-13. The majority of patients with G12 and available vaccination histories were unvaccinated. There was no genomic evidence to indicate that the G12 strains in St. Louis had evolved to escape vaccine protection. Our work emphasizes the need for continued surveillance.
AB - Background. Rotaviruses are a leading cause of gastroenteritis. Rotavirus vaccination has dramatically reduced rotavirus occurrence; however, we have noticed mild to moderate recurrences in the St. Louis area in alternate years. In 2013, we found rotavirus genotype G12 to be the dominant strain in the St. Louis region. In this study, we again determined the distribution of genotypes and ascertained vaccine history in patients infected with rotavirus G12 during the 2014-15 season. Methods. Samples submitted to the St. Louis Children's Hospital Microbiology Laboratory were tested for rotavirus using an antigen assay. We determined the VP7 genotype using amplicon sequence analysis. We determined genome sequences using high-throughput sequencing. We evaluated rotavirus immunization records when available. Results. Of 30 typed viruses from 2014-15, 29 were G12 (97%). Whole-genome sequencing revealed few changes from G12 viruses analyzed in 2012-13. VP4 and VP7 sequences were > 99% identical to previously sequenced G12 strains from St. Louis, and immune epitopes were conserved. Vaccination histories were available from 17 patients. Of these, 4 had been vaccinated, 3 had received incomplete vaccination or had a vaccine history that could not be confirmed, and 10 had not been vaccinated. Conclusions. G12 re-emerged as the predominant rotavirus genotype in 2014-15, comprising a higher percentage of cases than in 2012-13. The majority of patients with G12 and available vaccination histories were unvaccinated. There was no genomic evidence to indicate that the G12 strains in St. Louis had evolved to escape vaccine protection. Our work emphasizes the need for continued surveillance.
KW - Capture
KW - G12
KW - Rotavirus
KW - Sequencing
KW - Surveillance
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U2 - 10.1093/jpids/piw065
DO - 10.1093/jpids/piw065
M3 - Article
C2 - 27988496
AN - SCOPUS:85039162620
SN - 2048-7193
VL - 6
SP - 346
EP - 351
JO - Journal of the Pediatric Infectious Diseases Society
JF - Journal of the Pediatric Infectious Diseases Society
IS - 4
ER -