Reproducibility of high-resolution optical coherence tomography in multiple sclerosis

Stephanie B. Syc, Christina V. Warner, Girish S. Hiremath, Sheena K. Farrell, John N. Ratchford, Amy Conger, Teresa Frohman, Gary Cutter, Laura J. Balcer, Elliot Frohman, Peter A. Calabresi

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


Optical coherence tomography (OCT) is a non-invasive method to quantify neurodegeneration as an outcome in multiple sclerosis clinical trials; however, no data exist on Cirrus spectral domain optical coherence tomography (SD-OCT) reproducibility in patients with multiple sclerosis. The objective of this study was to determine the protocol for achieving optimal inter-visit, inter-rater, and intra-rater reproducibility for studies performed on healthy controls and multiple sclerosis patients utilizing novel high-definition SD-OCT. This is a prospective study of inter-visit, inter-rater, and intra-rater reproducibility in multiple sclerosis patients (n = 58) and healthy controls (n = 32) on Cirrus-HD SD-OCT. Excellent reproducibility of average and quadrantic retinal nerve fiber layer (RNFL) thickness values, average macular thickness (AMT), and total macular volume (TMV) [measured by intraclass correlation coefficient (ICC)] was found for inter-visit (healthy controls: mean RNFL = 0.97, quadrant range = 0.92-0.97, AMT = 0.97, TMV = 0.92), inter-rater (MS: mean RNFL = 0.97, quadrant = 0.94-0.98, AMT = 0.99, TMV = 0.96; healthy controls: mean RNFL = 0.97, quadrant = 0.94-0.97, AMT = 0.98, TMV = 0.99), and intra-rater (MS patients: mean RNFL = 0.99, quadrant = 0.83-0.99, AMT = 0.97, TMV = 0.98) reproducibility. The reproducibility of retinal measures derived by Cirrus HD-OCT, especially quadrantic values, is excellent. Specific procedures for OCT acquisition and analysis of retinal imaging metrics using SD-OCT technology may improve the application of this novel technology in multiple sclerosis.

Original languageEnglish (US)
Pages (from-to)829-839
Number of pages11
JournalMultiple Sclerosis
Issue number7
StatePublished - Jul 2010


  • Demyelinating disease (CNS)
  • axonal loss
  • multiple sclerosis
  • neuro-ophthalmology
  • optic nerve
  • outcome measurement

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology


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