TY - JOUR
T1 - Reproducibility and reuse of adaptive immune receptor repertoire data
AU - Breden, Felix
AU - Luning Prak, Eline T.
AU - Peters, Bjoern
AU - Rubelt, Florian
AU - Schramm, Chaim A.
AU - Busse, Christian E.
AU - Vander Heiden, Jason A.
AU - Christley, Scott
AU - Bukhari, Syed Ahmad Chan
AU - Thorogood, Adrian
AU - Matsen, Frederick A.
AU - Wine, Yariv
AU - Laserson, Uri
AU - Klatzmann, David
AU - Douek, Daniel C.
AU - Lefranc, Marie Paule
AU - Collins, Andrew M.
AU - Bubela, Tania
AU - Kleinstein, Steven H.
AU - Watson, Corey T.
AU - Cowell, Lindsay G.
AU - Scott, Jamie K.
AU - Kepler, Thomas B.
N1 - Publisher Copyright:
© 2017 Breden, Luning Prak, Peters, Rubelt, Schramm, Busse, Vander Heiden, Christley, Bukhari, Thorogood, Matsen IV, Wine, Laserson, Klatzmann, Douek, Lefranc, Collins, Bubela, Kleinstein, Watson, Cowell, Scott and Kepler.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - High-throughput sequencing (HTS) of immunoglobulin (B-cell receptor, antibody) and T-cell receptor repertoires has increased dramatically since the technique was introduced in 2009 (1-3). This experimental approach explores the maturation of the adaptive immune system and its response to antigens, pathogens, and disease conditions in exquisite detail. It holds significant promise for diagnostic and therapy-guiding applications. New technology often spreads rapidly, sometimes more rapidly than the understanding of how to make the products of that technology reliable, reproducible, or usable by others. As complex technologies have developed, scientific communities have come together to adopt common standards, protocols, and policies for generating and sharing data sets, such as the MIAME protocols developed for microarray experiments. The Adaptive Immune Receptor Repertoire (AIRR) Community formed in 2015 to address similar issues for HTS data of immune repertoires. The purpose of this perspective is to provide an overview of the AIRR Community's founding principles and present the progress that the AIRR Community has made in developing standards of practice and data sharing protocols. Finally, and most important, we invite all interested parties to join this effort to facilitate sharing and use of these powerful data sets (join@airr-community.org).
AB - High-throughput sequencing (HTS) of immunoglobulin (B-cell receptor, antibody) and T-cell receptor repertoires has increased dramatically since the technique was introduced in 2009 (1-3). This experimental approach explores the maturation of the adaptive immune system and its response to antigens, pathogens, and disease conditions in exquisite detail. It holds significant promise for diagnostic and therapy-guiding applications. New technology often spreads rapidly, sometimes more rapidly than the understanding of how to make the products of that technology reliable, reproducible, or usable by others. As complex technologies have developed, scientific communities have come together to adopt common standards, protocols, and policies for generating and sharing data sets, such as the MIAME protocols developed for microarray experiments. The Adaptive Immune Receptor Repertoire (AIRR) Community formed in 2015 to address similar issues for HTS data of immune repertoires. The purpose of this perspective is to provide an overview of the AIRR Community's founding principles and present the progress that the AIRR Community has made in developing standards of practice and data sharing protocols. Finally, and most important, we invite all interested parties to join this effort to facilitate sharing and use of these powerful data sets (join@airr-community.org).
KW - Antibodies
KW - B-cell receptors
KW - Community standards
KW - Data sharing
KW - High-throughput sequencing
KW - Immunogenetics
KW - Immunoglobulins
KW - T-cell receptors
UR - http://www.scopus.com/inward/record.url?scp=85032719118&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032719118&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2017.01418
DO - 10.3389/fimmu.2017.01418
M3 - Article
C2 - 29163494
AN - SCOPUS:85032719118
SN - 1664-3224
VL - 8
JO - Frontiers in immunology
JF - Frontiers in immunology
IS - NOV
M1 - 1418
ER -