Replica plating of mammalian cells using low melt agarose

Shobhana Natarajan; Tracy T. Chow; Jerry W. Shay; Woodring E. Wright

doi:10.1007/s10616-007-9071-6

Replica plating of mammalian cells using low melt agarose

Shobhana Natarajan, Tracy T. Chow, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We have developed a technique to replica plate mammalian cells grown on plastic dishes using low melt agarose. This method is simpler than previously described methods that use polyester membranes to grow and transfer cells. We have tested the effectiveness of this technique on normal and immortal cell lines and have found that we can transfer cells with an efficiency of 80-90%. We have used this technique to rapidly screen clones for insertion of a lentivirally encoded gene without a selectable marker.

Original language	English (US)
Pages (from-to)	145-147
Number of pages	3
Journal	Cytotechnology
Volume	54
Issue number	3
DOIs	https://doi.org/10.1007/s10616-007-9071-6
State	Published - Jul 2007

Keywords

Cell culture
Replica plating
Ring cloning
Screening
Transfection

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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AU - Chow, Tracy T.

AU - Shay, Jerry W.

AU - Wright, Woodring E.

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AB - We have developed a technique to replica plate mammalian cells grown on plastic dishes using low melt agarose. This method is simpler than previously described methods that use polyester membranes to grow and transfer cells. We have tested the effectiveness of this technique on normal and immortal cell lines and have found that we can transfer cells with an efficiency of 80-90%. We have used this technique to rapidly screen clones for insertion of a lentivirally encoded gene without a selectable marker.

KW - Cell culture

KW - Replica plating

KW - Ring cloning

KW - Screening

KW - Transfection

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Replica plating of mammalian cells using low melt agarose

Abstract

Keywords

ASJC Scopus subject areas

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