Abstract
Background: Alterations in high-density lipoprotein (HDL) subfractions, especially in the HDL2b subfraction, have been reported in type 2 diabetes mellitus (T2DM). However, new methods for convenient and reliable quantitation of HDL2b are yet to be developed. Methods: Thirty-eight patients with T2DM were enrolled and age-, sex- and body mass index (BMI)-matched controls were selected from the same population. A microfluidic chip method was employed to analyse serum HDL subfractions. Results: The microfluidic chip method revealed a significant reduction in HDL2b and its ratio to total HDL in T2DM patients. There was a reverse correlation for total HDL and HDL2b, and its ratio with triglycerides, homeostasis model assessment-insulin sensitivity index (HOMA-IS) and insulin resistance index (HOMA-IR). Conclusions: We have shown a reduction of HDL2b and its ratio to total HDL by a novel chip method in T2DM patients. The significant correlation between HDL2b and HOMA-IS and HOMA-IR may have further predictive value in clinical utility.
Original language | English (US) |
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Pages (from-to) | 619-623 |
Number of pages | 5 |
Journal | Biomarkers |
Volume | 14 |
Issue number | 8 |
DOIs | |
State | Published - Dec 2009 |
Keywords
- Bioanalyser
- HDL subfractions
- Microfluidic chip
- Type 2 diabetes
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
- Health, Toxicology and Mutagenesis