Reconstitution of catecholamine-sensitive adenylate cyclase activity: interaction of solubilized components with receptor-replete membranes

Membranes of mouse L cells which contain adenylate cyclase [ATP pyrophosphate-lyase (cyclizing)] but lack β adrenergic receptors were solubilized with Lubrol 12A9. Addition of such adenylate cyclase containing extracts to β adrenergic receptor replete membranes from adenylate cyclase deficient S49 lymphoma cells results in the production of a catecholamine sensitive adenylate cyclase system. The effects of β adrenergic agonists and antagonists on the reconstituted system reproduce those that are characteristic of the wild type S49 lymphoma cell. The uncoupled variant of the S49 lymphoma contains adenylate cyclase, but donor extracts from this clone fail to reconstitute the hormone sensitive enzyme activity when added to adenylate cyclase deficient membranes. Thus, the uncoupled and adenylate cyclase deficient variants of the S49 cell are not complementary.