TY - JOUR
T1 - Recent insights in phosphatidylinositol signaling
AU - Majerus, Philip W.
AU - Ross, Theodora S.
AU - Cunningham, Thomas W.
AU - Caldwell, Kevin K.
AU - Jefferson, Anne Bennett
AU - Bansal, Vinay S.
N1 - Funding Information:
This research was supported by grant HL 14147 (Specialized Center for Research in Thrombosis), grant I-IL 16634, and training grant HL 07066 from the National Institutes of Health.
PY - 1990/11/2
Y1 - 1990/11/2
N2 - Studies of phosphatidylinositol signaling pathways are entering a new phase in which molecular genetic techniques are providing powerful tools to dissect the functions of various metabolites and pathways. Studies with phospholipase C are most advanced and clearly indicate that phosphatidylinositol turnover is critical for vision in Drosophila and cell proliferation in various cultured cells. Expression of cDNA constructs and microinjection of PLC or antibodies against it clearly establish a role for PtdIns signaling distinct from its role in calcium mobilization and protein kinase C activation. The importance of inositol cyclic phosphates is also beginning to be realized from the study of cyclic hydrolase using similar techniques. Elucidation of the function of the 3-phosphate inositol phospholipid pathway awaits similar studies. The recent cDNA cloning of inositol monophosphatase (Diehl et al., 1990), Ins(1,4,5)P3 3-kinase (Choi et al., 1990), and inositol polyphosphate 1-phosphatase (York and Majerus, 1991) should provide tools to define further the cell biology of the phosphatidylinositol signaling pathway.
AB - Studies of phosphatidylinositol signaling pathways are entering a new phase in which molecular genetic techniques are providing powerful tools to dissect the functions of various metabolites and pathways. Studies with phospholipase C are most advanced and clearly indicate that phosphatidylinositol turnover is critical for vision in Drosophila and cell proliferation in various cultured cells. Expression of cDNA constructs and microinjection of PLC or antibodies against it clearly establish a role for PtdIns signaling distinct from its role in calcium mobilization and protein kinase C activation. The importance of inositol cyclic phosphates is also beginning to be realized from the study of cyclic hydrolase using similar techniques. Elucidation of the function of the 3-phosphate inositol phospholipid pathway awaits similar studies. The recent cDNA cloning of inositol monophosphatase (Diehl et al., 1990), Ins(1,4,5)P3 3-kinase (Choi et al., 1990), and inositol polyphosphate 1-phosphatase (York and Majerus, 1991) should provide tools to define further the cell biology of the phosphatidylinositol signaling pathway.
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U2 - 10.1016/0092-8674(90)90442-H
DO - 10.1016/0092-8674(90)90442-H
M3 - Review article
C2 - 2225061
AN - SCOPUS:0025114863
SN - 0092-8674
VL - 63
SP - 459
EP - 465
JO - Cell
JF - Cell
IS - 3
ER -