@article{780f21cdc2b7401cb26366a31402c45e,
title = "Rapid senescence induced by overexpression of p53 in NIH3T3 cells",
abstract = "An NIH3T3 cell line which overexpresses temperature-sensitive p53Val135 was constructed by introduction of p53Val135 gene. It exhibited rapidly characteristic morphological and biochemical alterations related to replicative senescence when being cultured in 32 °C. We suggested that the overexpression of p53 activated probably the onset of senescence in NIH3T3 cells, which induced a rapid cellular senescence.",
keywords = "NIH3T3, Overexpression, P53, P53Val135, Senescence",
author = "Jiancheng Hu and Ying Lei and Xia Hong and Zhigang Lu and Zhonghe Zhai",
note = "Funding Information: Acknowledgements We thank Prof. Moshe Oren in Weizman Institute of Israel for providing the plasmids PLXSNp53 NIH3T3 and PLXSN, and Dr. Wu Xiaobing with the Chinese Academy of Medical Sciences for providing packaging cell line GP+E-86. Also we thank Dr. Tao Wei, Zhao Yun, Liu Xiaoling and others in our lab for their help. This work was supported by the National Natural Science Foundation of China (Grant No. 39300075) and the National Basic Sciences Research Program (Grant No. G19990539).",
year = "2001",
doi = "10.1007/BF03182827",
language = "English (US)",
volume = "46",
pages = "653--656",
journal = "Chinese Science Bulletin",
issn = "1001-6538",
publisher = "Springer Science + Business Media",
number = "8",
}