Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the golgi structure

Yijun Zhang, Joachim Seemann

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


GRASP55 and GRASP65 have been implicated in stacking of Golgi cisternae and lateral linking of stacks within the Golgi ribbon. However, RNAi or gene knockout approaches to dissect their respective roles have often resulted in conflicting conclusions. Here, we gene-edited GRASP55 and/or GRASP65 with a degron tag in human fibroblasts, allowing for induced rapid degradation by the proteasome. We show that acute depletion of either GRASP55 or GRASP65 does not affect the Golgi ribbon, while chronic degradation of GRASP55 disrupts lateral connectivity of the ribbon. Acute double depletion of both GRASPs coincides with the loss of the vesicle tethering proteins GM130, p115, and Golgin-45 from the Golgi and compromises ribbon linking. Furthermore, GRASP55 and/or GRASP65 is not required for maintaining stacks or de novo assembly of stacked cisternae at the end of mitosis. These results demonstrate that both GRASPs are dispensable for Golgi stacking but are involved in maintaining the integrity of the Golgi ribbon together with GM130 and Golgin-45.

Original languageEnglish (US)
Article numbere202007052
JournalJournal of Cell Biology
Issue number1
StatePublished - Jan 4 2021


  • Organelles

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the golgi structure'. Together they form a unique fingerprint.

Cite this