Radioimmunoassay of digitonin

Gary Brooker; John W. Fleming; Elliott M. Ross

doi:10.1016/0005-2736(82)90032-3

Radioimmunoassay of digitonin

Gary Brooker, John W. Fleming, Elliott M. Ross

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with ¹²⁵I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 μg/ml (15 μM) digitonin. The assay is insensitive to interference by cholesterol or other cell membrane constituents and is useable above and below the critical micelle concentration of digitonin. It should be useful for the monitoring of digitonin concentrations in solubilized biochemical preparations.

Original language	English (US)
Pages (from-to)	39-43
Number of pages	5
Journal	BBA - Biomembranes
Volume	685
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(82)90032-3
State	Published - Feb 8 1982

Keywords

Digitonin assay
Radioimmunoassay

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(82)90032-3

Cite this

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title = "Radioimmunoassay of digitonin",

abstract = "We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 μg/ml (15 μM) digitonin. The assay is insensitive to interference by cholesterol or other cell membrane constituents and is useable above and below the critical micelle concentration of digitonin. It should be useful for the monitoring of digitonin concentrations in solubilized biochemical preparations.",

keywords = "Digitonin assay, Radioimmunoassay",

author = "Gary Brooker and Fleming, {John W.} and Ross, {Elliott M.}",

note = "Funding Information: This work was supported by USPHS grants GM26445 (to E.M.R.), HL15985, AM17042, HL19242 (to G.B.), postdoctoral fellowship GM07306 (to J.W.F.), and Diabetes Research and Training Center grant AM22125. E.M.R. is an Established Investigator of the American Heart Association.",

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doi = "10.1016/0005-2736(82)90032-3",

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T1 - Radioimmunoassay of digitonin

AU - Brooker, Gary

AU - Fleming, John W.

AU - Ross, Elliott M.

N1 - Funding Information: This work was supported by USPHS grants GM26445 (to E.M.R.), HL15985, AM17042, HL19242 (to G.B.), postdoctoral fellowship GM07306 (to J.W.F.), and Diabetes Research and Training Center grant AM22125. E.M.R. is an Established Investigator of the American Heart Association.

PY - 1982/2/8

Y1 - 1982/2/8

N2 - We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 μg/ml (15 μM) digitonin. The assay is insensitive to interference by cholesterol or other cell membrane constituents and is useable above and below the critical micelle concentration of digitonin. It should be useful for the monitoring of digitonin concentrations in solubilized biochemical preparations.

AB - We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 μg/ml (15 μM) digitonin. The assay is insensitive to interference by cholesterol or other cell membrane constituents and is useable above and below the critical micelle concentration of digitonin. It should be useful for the monitoring of digitonin concentrations in solubilized biochemical preparations.

KW - Digitonin assay

KW - Radioimmunoassay

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JF - BBA - Biomembranes

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Radioimmunoassay of digitonin

Abstract

Keywords

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