Abstract
Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.
Original language | English (US) |
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Pages (from-to) | 189-196 |
Number of pages | 8 |
Journal | Methods in Molecular Biology |
Volume | 1422 |
DOIs | |
State | Published - 2016 |
Keywords
- Adoptive transfer
- Group 3 Innate lymphoid cells
- Intestine
- Purification
- Rag1
ASJC Scopus subject areas
- Molecular Biology
- Genetics