Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound

Mohandas Dhaneesha; Md Umar; Titus Susan Merlin; Kottekkatu Padinchati Krishnan; Vrinda Sukumaran; Rupesh Kumar Sinha; Abdulaziz Anas; Peng Fu; John Bryan MacMillan; Thavarool Puthiyedathu Sajeevan

doi:10.1007/s10482-020-01490-7

Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound

Mohandas Dhaneesha, Md Umar, Titus Susan Merlin, Kottekkatu Padinchati Krishnan, Vrinda Sukumaran, Rupesh Kumar Sinha, Abdulaziz Anas, Peng Fu, John Bryan MacMillan, Thavarool Puthiyedathu Sajeevan

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10 Scopus citations

Abstract

Herein we report the isolation of a novel actinomycete, strain MCCB 268^T, from the sediment sample collected from a high Arctic fjord Kongsfjorden. MCCB 268^T showed greater than 97% 16S rRNA gene sequence similarity with those of Pseudonocardia konjuensis LM 157^T (98.06%), Pseudonocardia soli NW8-21 (97.22%) Pseudonocardia endophytica YIM 56035 (97.08%) and Pseudonocardia nantongensis KLBMP 1282 (97.34%) showing that the strain should be assigned to the genus Pseudonocardia. DNA–DNA hybridization with Pseudonocardia konjuensis LM 157^T showed only 41.5% relatedness to strain MCCB 268^T. The whole genome of the strain MCCB 268^T was sequenced. Whole-genome average nucleotide identity, dDDH (%) and genome tree analysis demonstrated that strain significantly differed from other Pseudonocardia species. The G + C content was 70.5 mol%. MCCB 268^T exhibited in vitro cytotoxicity and through bioassay guided fractionation followed by HPLC separation a cytotoxic compound (I) was isolated. The compound (I) was identified as 1-acetyl-β-carboline through NMR spectra and high-resolution mass spectrometry. Compound (I) showed cytotoxicity against lung cancer cell line and mode of anticancer activity was found to be through the induction of apoptosis. Based on the genotypic and phenotypic features, MCCB 268^T ought to be classified as a novel species under the genus Pseudonocardia for which the name Pseudonocardia cytotoxica sp. nov. is proposed (= CCUG72333^T = JCM32718^T).

Original language	English (US)
Pages (from-to)	23-35
Number of pages	13
Journal	Antonie van Leeuwenhoek
Volume	114
Issue number	1
DOIs	https://doi.org/10.1007/s10482-020-01490-7
State	Published - Jan 2021

Keywords

Actinomycetes
Apoptosis
Arctic
Cytotoxic
Pseudonocardia
β-Carboline

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1007/s10482-020-01490-7

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title = "Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound",

abstract = "Herein we report the isolation of a novel actinomycete, strain MCCB 268T, from the sediment sample collected from a high Arctic fjord Kongsfjorden. MCCB 268T showed greater than 97% 16S rRNA gene sequence similarity with those of Pseudonocardia konjuensis LM 157T (98.06%), Pseudonocardia soli NW8-21 (97.22%) Pseudonocardia endophytica YIM 56035 (97.08%) and Pseudonocardia nantongensis KLBMP 1282 (97.34%) showing that the strain should be assigned to the genus Pseudonocardia. DNA–DNA hybridization with Pseudonocardia konjuensis LM 157T showed only 41.5% relatedness to strain MCCB 268T. The whole genome of the strain MCCB 268T was sequenced. Whole-genome average nucleotide identity, dDDH (%) and genome tree analysis demonstrated that strain significantly differed from other Pseudonocardia species. The G + C content was 70.5 mol%. MCCB 268T exhibited in vitro cytotoxicity and through bioassay guided fractionation followed by HPLC separation a cytotoxic compound (I) was isolated. The compound (I) was identified as 1-acetyl-β-carboline through NMR spectra and high-resolution mass spectrometry. Compound (I) showed cytotoxicity against lung cancer cell line and mode of anticancer activity was found to be through the induction of apoptosis. Based on the genotypic and phenotypic features, MCCB 268T ought to be classified as a novel species under the genus Pseudonocardia for which the name Pseudonocardia cytotoxica sp. nov. is proposed (= CCUG72333T = JCM32718T).",

keywords = "Actinomycetes, Apoptosis, Arctic, Cytotoxic, Pseudonocardia, β-Carboline",

author = "Mohandas Dhaneesha and Md Umar and Merlin, {Titus Susan} and Krishnan, {Kottekkatu Padinchati} and Vrinda Sukumaran and Sinha, {Rupesh Kumar} and Abdulaziz Anas and Peng Fu and MacMillan, {John Bryan} and Sajeevan, {Thavarool Puthiyedathu}",

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doi = "10.1007/s10482-020-01490-7",

language = "English (US)",

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T1 - Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound

AU - Dhaneesha, Mohandas

AU - Umar, Md

AU - Merlin, Titus Susan

AU - Krishnan, Kottekkatu Padinchati

AU - Sukumaran, Vrinda

AU - Sinha, Rupesh Kumar

AU - Anas, Abdulaziz

AU - Fu, Peng

AU - MacMillan, John Bryan

AU - Sajeevan, Thavarool Puthiyedathu

PY - 2021/1

Y1 - 2021/1

N2 - Herein we report the isolation of a novel actinomycete, strain MCCB 268T, from the sediment sample collected from a high Arctic fjord Kongsfjorden. MCCB 268T showed greater than 97% 16S rRNA gene sequence similarity with those of Pseudonocardia konjuensis LM 157T (98.06%), Pseudonocardia soli NW8-21 (97.22%) Pseudonocardia endophytica YIM 56035 (97.08%) and Pseudonocardia nantongensis KLBMP 1282 (97.34%) showing that the strain should be assigned to the genus Pseudonocardia. DNA–DNA hybridization with Pseudonocardia konjuensis LM 157T showed only 41.5% relatedness to strain MCCB 268T. The whole genome of the strain MCCB 268T was sequenced. Whole-genome average nucleotide identity, dDDH (%) and genome tree analysis demonstrated that strain significantly differed from other Pseudonocardia species. The G + C content was 70.5 mol%. MCCB 268T exhibited in vitro cytotoxicity and through bioassay guided fractionation followed by HPLC separation a cytotoxic compound (I) was isolated. The compound (I) was identified as 1-acetyl-β-carboline through NMR spectra and high-resolution mass spectrometry. Compound (I) showed cytotoxicity against lung cancer cell line and mode of anticancer activity was found to be through the induction of apoptosis. Based on the genotypic and phenotypic features, MCCB 268T ought to be classified as a novel species under the genus Pseudonocardia for which the name Pseudonocardia cytotoxica sp. nov. is proposed (= CCUG72333T = JCM32718T).

AB - Herein we report the isolation of a novel actinomycete, strain MCCB 268T, from the sediment sample collected from a high Arctic fjord Kongsfjorden. MCCB 268T showed greater than 97% 16S rRNA gene sequence similarity with those of Pseudonocardia konjuensis LM 157T (98.06%), Pseudonocardia soli NW8-21 (97.22%) Pseudonocardia endophytica YIM 56035 (97.08%) and Pseudonocardia nantongensis KLBMP 1282 (97.34%) showing that the strain should be assigned to the genus Pseudonocardia. DNA–DNA hybridization with Pseudonocardia konjuensis LM 157T showed only 41.5% relatedness to strain MCCB 268T. The whole genome of the strain MCCB 268T was sequenced. Whole-genome average nucleotide identity, dDDH (%) and genome tree analysis demonstrated that strain significantly differed from other Pseudonocardia species. The G + C content was 70.5 mol%. MCCB 268T exhibited in vitro cytotoxicity and through bioassay guided fractionation followed by HPLC separation a cytotoxic compound (I) was isolated. The compound (I) was identified as 1-acetyl-β-carboline through NMR spectra and high-resolution mass spectrometry. Compound (I) showed cytotoxicity against lung cancer cell line and mode of anticancer activity was found to be through the induction of apoptosis. Based on the genotypic and phenotypic features, MCCB 268T ought to be classified as a novel species under the genus Pseudonocardia for which the name Pseudonocardia cytotoxica sp. nov. is proposed (= CCUG72333T = JCM32718T).

KW - Actinomycetes

KW - Apoptosis

KW - Arctic

KW - Cytotoxic

KW - Pseudonocardia

KW - β-Carboline

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SN - 0003-6072

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JO - Antonie van Leeuwenhoek

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ER -

Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound

Abstract

Keywords

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