@inbook{e806fcea0c614096b70f8280942fa06c,
title = "Protein arrays III: Reverse-phase protein arrays",
abstract = "The reverse-phase protein array (RPPA) is to use highly specific antibodies to interrogate pan or posttranslationally modified protein targets, such as phosphorylated proteins, particularly the proteins involved in cell signaling pathways. In this protocol we will cover the preparation of cell (or tissue) lysates, sample printing, antibody validation, antibody interrogation, signal amplification steps, imaging and data analysis. In this protocol, colorimetric catalyzed signal amplification (CSA) chemistry, fluorescence and near-infrared (NIR) based detection methods will be described.",
keywords = "Antibody validation, Cell signaling pathways, Near-infrared (NIR) fluorescence, Reverse-phase protein array",
author = "Yulin Yuan and Xia Hong and Lin, {Zuan Tao} and Hongting Wang and Mikala Heon and Tianfu Wu",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-7231-9_21",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "279--289",
booktitle = "Methods in Molecular Biology",
}