Protective effect of growth hormone on neuronal apoptosis after hypoxia-ischemia in the neonatal rat brain

Dong Hoon Shin; Eunju Lee; Jong Wan Kim; Bum Sun Kwon; Mi Kyung Jung; Youn Hee Jee; Jaehyup Kim; Su Ryeon Bae; Young Pyo Chang

doi:10.1016/j.neulet.2003.09.070

Protective effect of growth hormone on neuronal apoptosis after hypoxia-ischemia in the neonatal rat brain

Dong Hoon Shin, Eunju Lee, Jong Wan Kim, Bum Sun Kwon, Mi Kyung Jung, Youn Hee Jee, Jaehyup Kim, Su Ryeon Bae, Young Pyo Chang

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Recent studies have shown that growth hormone (GH) can reduce neuronal loss after hypoxic-ischemic injury (HI) in neonatal and juvenile rat brains. Here, we investigated whether GH exerts its neuroprotective role through an anti-apoptotic effect in neonatal rat brains damaged by severe HI. Gross and histological observations showed that the extent of brain damage was found to be reduced in GH-treated brain at E7 after injury. In a terminal transferase-mediated dUTP nick-end-labeling (TUNEL) study, TUNEL-positive apoptotic cells were localized only at the damaged region in animals treated with saline, which was confirmed by an electron microscopy. In an immunohistochemical study with anti-bcl-2, -bax, -bad, -neuronal nitric oxide synthase (nNOS), -inducible NOS (iNOS) and -endothelial NOS (eNOS) antibodies, we observed that bax, bad, iNOS and eNOS were elevated in the saline-treated group. This study thus suggests that the protective role of GH against HI injury is mediated thorough an anti-apoptotic effect, which offers the possibility of a GH application for the treatment of neonatal HI encephalopathy.

Original language	English (US)
Pages (from-to)	64-68
Number of pages	5
Journal	Neuroscience letters
Volume	354
Issue number	1
DOIs	https://doi.org/10.1016/j.neulet.2003.09.070
State	Published - Jan 2 2004
Externally published	Yes

Keywords

Bcl-2
Growth hormone
Hypoxia-ischemia
Nitric oxide synthase
Terminal transferase mediated dUTP nick end labeling

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2003.09.070

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title = "Protective effect of growth hormone on neuronal apoptosis after hypoxia-ischemia in the neonatal rat brain",

abstract = "Recent studies have shown that growth hormone (GH) can reduce neuronal loss after hypoxic-ischemic injury (HI) in neonatal and juvenile rat brains. Here, we investigated whether GH exerts its neuroprotective role through an anti-apoptotic effect in neonatal rat brains damaged by severe HI. Gross and histological observations showed that the extent of brain damage was found to be reduced in GH-treated brain at E7 after injury. In a terminal transferase-mediated dUTP nick-end-labeling (TUNEL) study, TUNEL-positive apoptotic cells were localized only at the damaged region in animals treated with saline, which was confirmed by an electron microscopy. In an immunohistochemical study with anti-bcl-2, -bax, -bad, -neuronal nitric oxide synthase (nNOS), -inducible NOS (iNOS) and -endothelial NOS (eNOS) antibodies, we observed that bax, bad, iNOS and eNOS were elevated in the saline-treated group. This study thus suggests that the protective role of GH against HI injury is mediated thorough an anti-apoptotic effect, which offers the possibility of a GH application for the treatment of neonatal HI encephalopathy.",

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author = "Shin, {Dong Hoon} and Eunju Lee and Kim, {Jong Wan} and Kwon, {Bum Sun} and Jung, {Mi Kyung} and Jee, {Youn Hee} and Jaehyup Kim and Bae, {Su Ryeon} and Chang, {Young Pyo}",

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AU - Shin, Dong Hoon

AU - Lee, Eunju

AU - Kim, Jong Wan

AU - Kwon, Bum Sun

AU - Jung, Mi Kyung

AU - Jee, Youn Hee

AU - Kim, Jaehyup

AU - Bae, Su Ryeon

AU - Chang, Young Pyo

PY - 2004/1/2

Y1 - 2004/1/2

N2 - Recent studies have shown that growth hormone (GH) can reduce neuronal loss after hypoxic-ischemic injury (HI) in neonatal and juvenile rat brains. Here, we investigated whether GH exerts its neuroprotective role through an anti-apoptotic effect in neonatal rat brains damaged by severe HI. Gross and histological observations showed that the extent of brain damage was found to be reduced in GH-treated brain at E7 after injury. In a terminal transferase-mediated dUTP nick-end-labeling (TUNEL) study, TUNEL-positive apoptotic cells were localized only at the damaged region in animals treated with saline, which was confirmed by an electron microscopy. In an immunohistochemical study with anti-bcl-2, -bax, -bad, -neuronal nitric oxide synthase (nNOS), -inducible NOS (iNOS) and -endothelial NOS (eNOS) antibodies, we observed that bax, bad, iNOS and eNOS were elevated in the saline-treated group. This study thus suggests that the protective role of GH against HI injury is mediated thorough an anti-apoptotic effect, which offers the possibility of a GH application for the treatment of neonatal HI encephalopathy.

AB - Recent studies have shown that growth hormone (GH) can reduce neuronal loss after hypoxic-ischemic injury (HI) in neonatal and juvenile rat brains. Here, we investigated whether GH exerts its neuroprotective role through an anti-apoptotic effect in neonatal rat brains damaged by severe HI. Gross and histological observations showed that the extent of brain damage was found to be reduced in GH-treated brain at E7 after injury. In a terminal transferase-mediated dUTP nick-end-labeling (TUNEL) study, TUNEL-positive apoptotic cells were localized only at the damaged region in animals treated with saline, which was confirmed by an electron microscopy. In an immunohistochemical study with anti-bcl-2, -bax, -bad, -neuronal nitric oxide synthase (nNOS), -inducible NOS (iNOS) and -endothelial NOS (eNOS) antibodies, we observed that bax, bad, iNOS and eNOS were elevated in the saline-treated group. This study thus suggests that the protective role of GH against HI injury is mediated thorough an anti-apoptotic effect, which offers the possibility of a GH application for the treatment of neonatal HI encephalopathy.

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KW - Terminal transferase mediated dUTP nick end labeling

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Protective effect of growth hormone on neuronal apoptosis after hypoxia-ischemia in the neonatal rat brain

Abstract

Keywords

ASJC Scopus subject areas

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