Production of L-Dopa by immobilized Pseudomonas sp

Gelin Wang, Xin Mao, Zhenrong Peng, Ping Shen

Research output: Contribution to journalArticlepeer-review


PVA (polyvinyl alcohol) and Ca-alginate gel were selected as the immobilization carrier to stabilize the tyrosinase in Pseudomonas sp. for repeat production of L-Dopa from L-tyrosine. Comparing with the free cells, the Ca-alginate and PVA immobilized cells shortened the fermentation cycle by 8 hours and 14 hours respectively. In semi-continuous fermentation, the two kinds of immobilized cells were able to use 5 and 14 times repeatedly, and the fermentation efficiency were 16% and 160% higher than that of free cells. It was found that the productivity of L-Dopa was apparently increased when the PVA immobilized cells were treated with heat or shaken in the physiological saline solution. Excessive amount of substrate was unfavorable for the repeat use of immobilized cells.

Original languageEnglish (US)
Pages (from-to)X21-484
JournalWuhan Daxue Xuebao/Journal of Wuhan University
Issue number4
StatePublished - Dec 1 1998


  • Immobilized cell
  • L-Dopa
  • PVA

ASJC Scopus subject areas

  • Management of Technology and Innovation


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