Production of endothelin-1 by rat cultured mesangial cells

Hisato Sakamoto; Sei Sasaki; Yukio Hirata; Taihei Imai; Kenji Ando; Takashi Ida; Takeshi Sakurai; Masashi Yanagisawa; Tomoh Masaki; Fumiaki Marumo

doi:10.1016/0006-291X(90)90354-P

Production of endothelin-1 by rat cultured mesangial cells

Hisato Sakamoto, Sei Sasaki, Yukio Hirata, Taihei Imai, Kenji Ando, Takashi Ida, Takeshi Sakurai, Masashi Yanagisawa, Tomoh Masaki, Fumiaki Marumo

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Abstract

We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A)⁺RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.

Original language	English (US)
Pages (from-to)	462-468
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)90354-P
State	Published - Jun 15 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{38b2b146e84e469fad759182bed4cff1,

title = "Production of endothelin-1 by rat cultured mesangial cells",

abstract = "We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A)+RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.",

author = "Hisato Sakamoto and Sei Sasaki and Yukio Hirata and Taihei Imai and Kenji Ando and Takashi Ida and Takeshi Sakurai and Masashi Yanagisawa and Tomoh Masaki and Fumiaki Marumo",

note = "Funding Information: ACKBOWEDGMMCS: This work was supported in part by a research grant from the Ministry of Health and Welfare(62A-1,63C-1) and by a Grant-in-Aid (N0.01480217, 01480286) for Scientific Research from the Ministry of Education, Science and Culture of Japan.",

year = "1990",

month = jun,

day = "15",

doi = "10.1016/0006-291X(90)90354-P",

language = "English (US)",

volume = "169",

pages = "462--468",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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T1 - Production of endothelin-1 by rat cultured mesangial cells

AU - Sakamoto, Hisato

AU - Sasaki, Sei

AU - Hirata, Yukio

AU - Imai, Taihei

AU - Ando, Kenji

AU - Ida, Takashi

AU - Sakurai, Takeshi

AU - Yanagisawa, Masashi

AU - Masaki, Tomoh

AU - Marumo, Fumiaki

N1 - Funding Information: ACKBOWEDGMMCS: This work was supported in part by a research grant from the Ministry of Health and Welfare(62A-1,63C-1) and by a Grant-in-Aid (N0.01480217, 01480286) for Scientific Research from the Ministry of Education, Science and Culture of Japan.

PY - 1990/6/15

Y1 - 1990/6/15

N2 - We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A)+RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.

AB - We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A)+RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.

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JO - Biochemical and Biophysical Research Communications

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Production of endothelin-1 by rat cultured mesangial cells

Abstract

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