Production of endothelin-1 by rat cultured mesangial cells

Hisato Sakamoto, Sei Sasaki, Yukio Hirata, Taihei Imai, Kenji Ando, Takashi Ida, Takeshi Sakurai, Masashi Yanagisawa, Tomoh Masaki, Fumiaki Marumo

Research output: Contribution to journalArticlepeer-review

108 Scopus citations


We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A)+RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.

Original languageEnglish (US)
Pages (from-to)462-468
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Jun 15 1990

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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