Abstract
Crystals of bacterially expressed plasminogen activator inhibitor (PAI‐1) suitable for X‐ray diffraction analysis have been obtained from 8% (w/v) PEG 1500, pH 8.25. The space group is P1, and the lattice constants are a = 82.17 Å, b = 47.82 Å, c = 62.89 Å, α = 90.00°, β = 106.90°, γ = 106.84°. The diffraction limit is 2.3 Å, and the unit cell contains two molecules of PAI‐1. The crystals contain latent PAI‐1 which can be partly reactivated by exposure to denaturants.
Original language | English (US) |
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Pages (from-to) | 225-227 |
Number of pages | 3 |
Journal | Proteins: Structure, Function, and Bioinformatics |
Volume | 9 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1991 |
Keywords
- N‐terminal
- crystallography
- latency
- methionine
- protein crystals
- tissue plasminogen activator
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Molecular Biology