TY - JOUR
T1 - Pre-existing anti-Salmonella vector immunity prevents the development of protective antigen-specific CD8 T-cell frequencies against murine listeriosis
AU - Sevil Domènech, Victòria E.
AU - Panthel, Klaus
AU - Meinel, Katrin M.
AU - Winter, Sebastian E.
AU - Rüssmann, Holger
N1 - Funding Information:
H.R. was supported by the Deutsche Forschungsgemeinschaft (Schwerpunktprogramm “Neue Vakzinierungsstrategien“, grant RU 838/1-3).
PY - 2007/10
Y1 - 2007/10
N2 - Our laboratory has focused its research on the use of the type III secretion system of Salmonella enterica serovar Typhimurium to translocate heterologous antigens directly into the cytosol of antigen-presenting cells. We have previously reported that the single oral immunization of mice with a recombinant Salmonella aroA/sptP mutant strain expressing the translocated Yersinia outer protein E fused to the immunodominant antigen p60 from Listeria monocytogenes in a type III-mediated fashion results in the efficient induction of p60-specific CD8 T cells and confers protection against a lethal Listeria challenge infection. In the present study, we determined whether pre-existing anti-Salmonella vector immunity influences the induction of p60-specific CD8 T cells and modulates protective immunity against listeriosis after oral vaccination with recombinant Salmonella. After single oral immunization, the Salmonella aroA/sptP double mutant strain was found to colonize spleens of mice for 21 days. In contrast, the period of colonization was significantly shortened to 6 days due to anti-Salmonella vector immunity after second oral immunization. The latter scenario led to the induction of low-level frequencies of antigen-specific CD8 T cells. Compared to the significantly higher numbers of p60-specific T lymphocytes elicited after single oral immunization, the low amount of Listeria-specific CD8 T cells did not confer protection against listeriosis.
AB - Our laboratory has focused its research on the use of the type III secretion system of Salmonella enterica serovar Typhimurium to translocate heterologous antigens directly into the cytosol of antigen-presenting cells. We have previously reported that the single oral immunization of mice with a recombinant Salmonella aroA/sptP mutant strain expressing the translocated Yersinia outer protein E fused to the immunodominant antigen p60 from Listeria monocytogenes in a type III-mediated fashion results in the efficient induction of p60-specific CD8 T cells and confers protection against a lethal Listeria challenge infection. In the present study, we determined whether pre-existing anti-Salmonella vector immunity influences the induction of p60-specific CD8 T cells and modulates protective immunity against listeriosis after oral vaccination with recombinant Salmonella. After single oral immunization, the Salmonella aroA/sptP double mutant strain was found to colonize spleens of mice for 21 days. In contrast, the period of colonization was significantly shortened to 6 days due to anti-Salmonella vector immunity after second oral immunization. The latter scenario led to the induction of low-level frequencies of antigen-specific CD8 T cells. Compared to the significantly higher numbers of p60-specific T lymphocytes elicited after single oral immunization, the low amount of Listeria-specific CD8 T cells did not confer protection against listeriosis.
KW - Anti-vector immunity
KW - CD8 T cell
KW - Listeriosis
KW - Protective Immunity
KW - Salmonella enterica serovar Typhimurium
KW - Type III secretion system
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U2 - 10.1016/j.micinf.2007.07.010
DO - 10.1016/j.micinf.2007.07.010
M3 - Article
C2 - 17913544
AN - SCOPUS:35648957194
SN - 1286-4579
VL - 9
SP - 1447
EP - 1453
JO - Microbes and Infection
JF - Microbes and Infection
IS - 12-13
ER -