TY - JOUR
T1 - PPAR-γ agonist protects against intestinal injury during necrotizing enterocolitis
AU - Baregamian, Naira
AU - Mourot, Joshua M.
AU - Ballard, Amie R.
AU - Evers, B. Mark
AU - Chung, Dai H.
N1 - Funding Information:
The authors thank Karen Martin for paper preparation. This work was supported by Grants, R01DK48498, R01DK61470, PO1DK35608 and T32DK07639, from the National Institutes of Health and by the Shriners Burns Hospital Grant (No. 8580).
PY - 2009/2/6
Y1 - 2009/2/6
N2 - Necrotizing enterocolitis (NEC) remains a lethal condition for many premature infants. Peroxisome proliferator-activated receptor-γ (PPAR-γ), a member of the nuclear hormone receptor family, has been shown to play a protective role in cellular inflammatory responses; however, its role in NEC is not clearly defined. We sought to examine the expression of PPAR-γ in the intestine using an ischemia-reperfusion (I/R) model of NEC, and to assess whether PPAR-γ agonist treatment would ameliorate I/R-induced gut injury. Swiss-Webster mice were randomized to receive sham (control) or I/R injury to the gut induced by transient occlusion of superior mesenteric artery for 45 min with variable periods of reperfusion. I/R injury resulted in early induction of PPAR-γ expression and activation of NF-κB in small intestine. Pretreatment with PPAR-γ agonist, 15d-PGJ2, attenuated intestinal NF-κB response and I/R-induced gut injury. Activation of PPAR-γ demonstrated a protective effect on small bowel during I/R-induced gut injury.
AB - Necrotizing enterocolitis (NEC) remains a lethal condition for many premature infants. Peroxisome proliferator-activated receptor-γ (PPAR-γ), a member of the nuclear hormone receptor family, has been shown to play a protective role in cellular inflammatory responses; however, its role in NEC is not clearly defined. We sought to examine the expression of PPAR-γ in the intestine using an ischemia-reperfusion (I/R) model of NEC, and to assess whether PPAR-γ agonist treatment would ameliorate I/R-induced gut injury. Swiss-Webster mice were randomized to receive sham (control) or I/R injury to the gut induced by transient occlusion of superior mesenteric artery for 45 min with variable periods of reperfusion. I/R injury resulted in early induction of PPAR-γ expression and activation of NF-κB in small intestine. Pretreatment with PPAR-γ agonist, 15d-PGJ2, attenuated intestinal NF-κB response and I/R-induced gut injury. Activation of PPAR-γ demonstrated a protective effect on small bowel during I/R-induced gut injury.
KW - Ischemia-reperfusion injury
KW - Necrotizing enterocolitis
KW - Nuclear factor-kappaB
KW - Peroxisome proliferator-activated receptor-γ
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U2 - 10.1016/j.bbrc.2008.11.155
DO - 10.1016/j.bbrc.2008.11.155
M3 - Article
C2 - 19114032
AN - SCOPUS:58149488639
SN - 0006-291X
VL - 379
SP - 423
EP - 427
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -