Abstract
The role of main factors influencing the rate of potato 5-lipoxygenase oxidation of linoleic acid was investigated. It was found that nonionic detergent lubrol PX inhibited the potato lipoxygenase. Optimal pH for the linoleic acid oxidation was 6.3 temperature--45 degrees C and substrate concentration--3 x 10(-4) M (if lubrol PX was 0.02%). It was shown that potato 5-lipoxygenase was allosteric enzyme which possessed positive cooperativity for linoleic acid. The Hill coefficient was calculated (n = 1.40 +/- 0.15) with S0.5 = 75 +/- 10 microM.
Translated title of the contribution | Potato 5-lipoxygenase. Kinetics of linoleic acid oxidation |
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Original language | Russian |
Pages (from-to) | 106-108 |
Number of pages | 3 |
Journal | Ukrainskii biokhimicheskii zhurnal |
Volume | 61 |
Issue number | 2 |
State | Published - Mar 1 1989 |
ASJC Scopus subject areas
- Biochemistry