Possibilities of using a miniature fluorescence microscope

Alexander Erofeev, Evgeny Gerasimov, Dmitry Barinov, Marina Bolsunovskaya, Ilya Bezprozvanny

Research output: Contribution to journalConference articlepeer-review

Abstract

The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, complexity and high cost of usage. The authors developed a unique algorithm of correlations building based on cross-correlation of neuronal signals and implemented the opportunity to import experiment's data in csv format. (as well as a utility for converting data from CNMF-E), which allows to import data from other sources. Special attention is paid to the methods of processing neural activity data: filtering the received frames from noise, compensating of image shifts and distortions.

Original languageEnglish (US)
Article number02005
JournalE3S Web of Conferences
Volume224
DOIs
StatePublished - Dec 23 2020
Event2020 Topical Problems of Agriculture, Civil and Environmental Engineering, TPACEE 2020 - Moscow, Russian Federation
Duration: Nov 25 2020Nov 27 2020

ASJC Scopus subject areas

  • General Environmental Science
  • General Energy
  • General Earth and Planetary Sciences

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