TY - JOUR
T1 - Positive selection of self- and alloreactive CD8+ T cells in Tap-1 mutant mice
AU - Aldrich, Carla J.
AU - Ljunggren, Hans Gustaf
AU - Van Kaer, Luc
AU - Ashton-Rickardt, Philip G.
AU - Tonegawa, Susumu
AU - Forman, James
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1994/7/5
Y1 - 1994/7/5
N2 - Mice with a homozygous deletion in their Tap-1 gene (-/- mice) express very low levels of cell membrane major histocompatibility complex class I molecules and have <1% peripheral CD8+ T cells. We show that these -/- mice but not their +/- littermates display strong primary syngeneic anti-H-2Kb and -Db-specific responses mediated by CD8+ T cells. These responses are augmented by in vivo priming. Further, -/- mice primed in vivo with H-2(d) alloantigens generate an anti-H-2(d) response which appears nearly as strong as that found in +/- littermates. Both -/- anti-H-2b and anti-H-2(d) T cells do not recognize target cells from Tap-1 -/- animals or Tap-2-deficient RMA- S cells. Thus, some CD8+ anti-self and alloreactive T cells can be selected in the absence of Tap proteins.
AB - Mice with a homozygous deletion in their Tap-1 gene (-/- mice) express very low levels of cell membrane major histocompatibility complex class I molecules and have <1% peripheral CD8+ T cells. We show that these -/- mice but not their +/- littermates display strong primary syngeneic anti-H-2Kb and -Db-specific responses mediated by CD8+ T cells. These responses are augmented by in vivo priming. Further, -/- mice primed in vivo with H-2(d) alloantigens generate an anti-H-2(d) response which appears nearly as strong as that found in +/- littermates. Both -/- anti-H-2b and anti-H-2(d) T cells do not recognize target cells from Tap-1 -/- animals or Tap-2-deficient RMA- S cells. Thus, some CD8+ anti-self and alloreactive T cells can be selected in the absence of Tap proteins.
KW - cytotoxic T lymphocytes
KW - major histocompatibility complex
KW - peptide transporters
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U2 - 10.1073/pnas.91.14.6525
DO - 10.1073/pnas.91.14.6525
M3 - Article
C2 - 8022816
AN - SCOPUS:0028300485
SN - 0027-8424
VL - 91
SP - 6525
EP - 6528
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 14
ER -