Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis

Wei Wang; Eric J. Hansen

doi:10.1016/j.plasmid.2006.03.002

Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis

Wei Wang, Eric J. Hansen

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The plasmid shuttle vector pWW102B is able to replicate in only a modest number of Moraxella catarrhalis strains. Plasmid pWW115, a spontaneous deletion mutant of pWW102B, was shown to lack both the pACYC184-derived origin of replication and the associated chloramphenicol-resistance gene but was able to replicate in every M. catarrhalis strain tested in this study, including one strain that had been previously refractory to all types of genetic manipulations. To test the utility of this plasmid, a M. catarrhalis gene encoding the UspA2 serum-resistance factor was cloned into pWW115 and the resultant recombinant plasmid was shown to confer serum-resistance on a serum-sensitive M. catarrhalis uspA2 mutant.

Original language	English (US)
Pages (from-to)	133-137
Number of pages	5
Journal	Plasmid
Volume	56
Issue number	2
DOIs	https://doi.org/10.1016/j.plasmid.2006.03.002
State	Published - Sep 2006

Keywords

Complementation
Moraxella catarrhalis
Plasmid

ASJC Scopus subject areas

Molecular Biology

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10.1016/j.plasmid.2006.03.002

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title = "Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis",

abstract = "The plasmid shuttle vector pWW102B is able to replicate in only a modest number of Moraxella catarrhalis strains. Plasmid pWW115, a spontaneous deletion mutant of pWW102B, was shown to lack both the pACYC184-derived origin of replication and the associated chloramphenicol-resistance gene but was able to replicate in every M. catarrhalis strain tested in this study, including one strain that had been previously refractory to all types of genetic manipulations. To test the utility of this plasmid, a M. catarrhalis gene encoding the UspA2 serum-resistance factor was cloned into pWW115 and the resultant recombinant plasmid was shown to confer serum-resistance on a serum-sensitive M. catarrhalis uspA2 mutant.",

keywords = "Complementation, Moraxella catarrhalis, Plasmid",

author = "Wei Wang and Hansen, {Eric J.}",

note = "Funding Information: This study was supported by US Public Health Service Grant No. AI36344 to E.J.H. The authors thank Ahmed S. Attia for performing the serum bactericidal activity assays. The authors also thank Drs. John Nelson, Anthony Campagnari, Steven Berk, Timothy Murphy, Christoph Aebi, George Syrogiannopoulos, and Merja Helminen for supplying the M. catarrhalis isolates used in this study. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2006",

month = sep,

doi = "10.1016/j.plasmid.2006.03.002",

language = "English (US)",

volume = "56",

pages = "133--137",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press Inc.",

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T1 - Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis

AU - Wang, Wei

AU - Hansen, Eric J.

N1 - Funding Information: This study was supported by US Public Health Service Grant No. AI36344 to E.J.H. The authors thank Ahmed S. Attia for performing the serum bactericidal activity assays. The authors also thank Drs. John Nelson, Anthony Campagnari, Steven Berk, Timothy Murphy, Christoph Aebi, George Syrogiannopoulos, and Merja Helminen for supplying the M. catarrhalis isolates used in this study. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2006/9

Y1 - 2006/9

N2 - The plasmid shuttle vector pWW102B is able to replicate in only a modest number of Moraxella catarrhalis strains. Plasmid pWW115, a spontaneous deletion mutant of pWW102B, was shown to lack both the pACYC184-derived origin of replication and the associated chloramphenicol-resistance gene but was able to replicate in every M. catarrhalis strain tested in this study, including one strain that had been previously refractory to all types of genetic manipulations. To test the utility of this plasmid, a M. catarrhalis gene encoding the UspA2 serum-resistance factor was cloned into pWW115 and the resultant recombinant plasmid was shown to confer serum-resistance on a serum-sensitive M. catarrhalis uspA2 mutant.

AB - The plasmid shuttle vector pWW102B is able to replicate in only a modest number of Moraxella catarrhalis strains. Plasmid pWW115, a spontaneous deletion mutant of pWW102B, was shown to lack both the pACYC184-derived origin of replication and the associated chloramphenicol-resistance gene but was able to replicate in every M. catarrhalis strain tested in this study, including one strain that had been previously refractory to all types of genetic manipulations. To test the utility of this plasmid, a M. catarrhalis gene encoding the UspA2 serum-resistance factor was cloned into pWW115 and the resultant recombinant plasmid was shown to confer serum-resistance on a serum-sensitive M. catarrhalis uspA2 mutant.

KW - Complementation

KW - Moraxella catarrhalis

KW - Plasmid

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SN - 0147-619X

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JO - Plasmid

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Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis

Abstract

Keywords

ASJC Scopus subject areas

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