TY - JOUR
T1 - Photocrosslinking approaches to interactome mapping
AU - Pham, Nam D.
AU - Parker, Randy B.
AU - Kohler, Jennifer J.
N1 - Funding Information:
We acknowledge financial support from the National Institutes of Health ( F30AG040909 , T32GM007062 , and R01GM090271 ) and thank all members of the Kohler lab for comments on the manuscript.
PY - 2013/2
Y1 - 2013/2
N2 - Photocrosslinking approaches can be used to map interactome networks within the context of living cells. Photocrosslinking methods rely on use of metabolic engineering or genetic code expansion to incorporate photocrosslinking analogs of amino acids or sugars into cellular biomolecules. Immunological and mass spectrometry techniques are used to analyze crosslinked complexes, thereby defining specific interactomes. Because photocrosslinking can be conducted in native, cellular settings, it can be used to define context-dependent interactions. Photocrosslinking methods are also ideally suited for determining interactome dynamics, mapping interaction interfaces, and identifying transient interactions in which intrinsically disordered proteins and glycoproteins engage. Here we discuss the application of cell-based photocrosslinking to the study of specific problems in immune cell signaling, transcription, membrane protein dynamics, nucleocytoplasmic transport, and chaperone-assisted protein folding.
AB - Photocrosslinking approaches can be used to map interactome networks within the context of living cells. Photocrosslinking methods rely on use of metabolic engineering or genetic code expansion to incorporate photocrosslinking analogs of amino acids or sugars into cellular biomolecules. Immunological and mass spectrometry techniques are used to analyze crosslinked complexes, thereby defining specific interactomes. Because photocrosslinking can be conducted in native, cellular settings, it can be used to define context-dependent interactions. Photocrosslinking methods are also ideally suited for determining interactome dynamics, mapping interaction interfaces, and identifying transient interactions in which intrinsically disordered proteins and glycoproteins engage. Here we discuss the application of cell-based photocrosslinking to the study of specific problems in immune cell signaling, transcription, membrane protein dynamics, nucleocytoplasmic transport, and chaperone-assisted protein folding.
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U2 - 10.1016/j.cbpa.2012.10.034
DO - 10.1016/j.cbpa.2012.10.034
M3 - Review article
C2 - 23149092
AN - SCOPUS:84875260407
SN - 1367-5931
VL - 17
SP - 90
EP - 101
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 1
ER -