Abstract
An aberrantly elevated expression of DNA polymerase ι (Pol ι) is significantly associated with poor prognosis of patients with esophageal squamous cell carcinoma (ESCC), yet the mechanisms behind this phenomenon remain obscure. Based on the RNA-Seq transcriptome and real-time PCR analysis, we identified ETS-1 as a candidate gene involved in Pol ι-mediated progression of ESCC. Wound-healing and transwell assay indicated that downregulation of ETS-1 attenuates Pol ι-mediated invasiveness of ESCC. Signaling pathway analysis showed that Pol ι enhances ETS-1 phosphorylation at threonine-38 through the Erk signaling pathway in ESCC cells. Kaplan–Meier analysis, based on 93 clinical tissue samples, revealed that ETS-1 phosphorylation at threonine-38 is associated with poor prognosis of ESCC patients. The present study thus demonstrates that phosphorylation of ETS-1 is a critical event in the Pol ι-induced invasion and metastasis of ESCC.
Original language | English (US) |
---|---|
Pages (from-to) | 2503-2510 |
Number of pages | 8 |
Journal | Cancer Science |
Volume | 108 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2017 |
Externally published | Yes |
Keywords
- DNA polymerase iota
- ETS-1 phosphorylation
- esophageal squamous cell carcinoma
- metastasis
- tumor invasion
ASJC Scopus subject areas
- Oncology
- Cancer Research