Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma

Chao He; Shuhua Wu; Aidi Gao; Ye Su; Han Min; Zeng Fu Shang; Jinchang Wu; Li Yang; Wei Qun Ding; Jundong Zhou

doi:10.1111/cas.13399

Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma

Chao He, Shuhua Wu, Aidi Gao, Ye Su, Han Min, Zeng Fu Shang, Jinchang Wu, Li Yang, Wei Qun Ding, Jundong Zhou

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

An aberrantly elevated expression of DNA polymerase ι (Pol ι) is significantly associated with poor prognosis of patients with esophageal squamous cell carcinoma (ESCC), yet the mechanisms behind this phenomenon remain obscure. Based on the RNA-Seq transcriptome and real-time PCR analysis, we identified ETS-1 as a candidate gene involved in Pol ι-mediated progression of ESCC. Wound-healing and transwell assay indicated that downregulation of ETS-1 attenuates Pol ι-mediated invasiveness of ESCC. Signaling pathway analysis showed that Pol ι enhances ETS-1 phosphorylation at threonine-38 through the Erk signaling pathway in ESCC cells. Kaplan–Meier analysis, based on 93 clinical tissue samples, revealed that ETS-1 phosphorylation at threonine-38 is associated with poor prognosis of ESCC patients. The present study thus demonstrates that phosphorylation of ETS-1 is a critical event in the Pol ι-induced invasion and metastasis of ESCC.

Original language	English (US)
Pages (from-to)	2503-2510
Number of pages	8
Journal	Cancer Science
Volume	108
Issue number	12
DOIs	https://doi.org/10.1111/cas.13399
State	Published - Dec 2017
Externally published	Yes

Keywords

DNA polymerase iota
ETS-1 phosphorylation
esophageal squamous cell carcinoma
metastasis
tumor invasion

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma",

abstract = "An aberrantly elevated expression of DNA polymerase ι (Pol ι) is significantly associated with poor prognosis of patients with esophageal squamous cell carcinoma (ESCC), yet the mechanisms behind this phenomenon remain obscure. Based on the RNA-Seq transcriptome and real-time PCR analysis, we identified ETS-1 as a candidate gene involved in Pol ι-mediated progression of ESCC. Wound-healing and transwell assay indicated that downregulation of ETS-1 attenuates Pol ι-mediated invasiveness of ESCC. Signaling pathway analysis showed that Pol ι enhances ETS-1 phosphorylation at threonine-38 through the Erk signaling pathway in ESCC cells. Kaplan–Meier analysis, based on 93 clinical tissue samples, revealed that ETS-1 phosphorylation at threonine-38 is associated with poor prognosis of ESCC patients. The present study thus demonstrates that phosphorylation of ETS-1 is a critical event in the Pol ι-induced invasion and metastasis of ESCC.",

keywords = "DNA polymerase iota, ETS-1 phosphorylation, esophageal squamous cell carcinoma, metastasis, tumor invasion",

author = "Chao He and Shuhua Wu and Aidi Gao and Ye Su and Han Min and Shang, {Zeng Fu} and Jinchang Wu and Li Yang and Ding, {Wei Qun} and Jundong Zhou",

note = "Publisher Copyright: {\textcopyright} 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.",

year = "2017",

month = dec,

doi = "10.1111/cas.13399",

language = "English (US)",

volume = "108",

pages = "2503--2510",

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T1 - Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma

AU - He, Chao

AU - Wu, Shuhua

AU - Gao, Aidi

AU - Su, Ye

AU - Min, Han

AU - Shang, Zeng Fu

AU - Wu, Jinchang

AU - Yang, Li

AU - Ding, Wei Qun

AU - Zhou, Jundong

PY - 2017/12

Y1 - 2017/12

N2 - An aberrantly elevated expression of DNA polymerase ι (Pol ι) is significantly associated with poor prognosis of patients with esophageal squamous cell carcinoma (ESCC), yet the mechanisms behind this phenomenon remain obscure. Based on the RNA-Seq transcriptome and real-time PCR analysis, we identified ETS-1 as a candidate gene involved in Pol ι-mediated progression of ESCC. Wound-healing and transwell assay indicated that downregulation of ETS-1 attenuates Pol ι-mediated invasiveness of ESCC. Signaling pathway analysis showed that Pol ι enhances ETS-1 phosphorylation at threonine-38 through the Erk signaling pathway in ESCC cells. Kaplan–Meier analysis, based on 93 clinical tissue samples, revealed that ETS-1 phosphorylation at threonine-38 is associated with poor prognosis of ESCC patients. The present study thus demonstrates that phosphorylation of ETS-1 is a critical event in the Pol ι-induced invasion and metastasis of ESCC.

AB - An aberrantly elevated expression of DNA polymerase ι (Pol ι) is significantly associated with poor prognosis of patients with esophageal squamous cell carcinoma (ESCC), yet the mechanisms behind this phenomenon remain obscure. Based on the RNA-Seq transcriptome and real-time PCR analysis, we identified ETS-1 as a candidate gene involved in Pol ι-mediated progression of ESCC. Wound-healing and transwell assay indicated that downregulation of ETS-1 attenuates Pol ι-mediated invasiveness of ESCC. Signaling pathway analysis showed that Pol ι enhances ETS-1 phosphorylation at threonine-38 through the Erk signaling pathway in ESCC cells. Kaplan–Meier analysis, based on 93 clinical tissue samples, revealed that ETS-1 phosphorylation at threonine-38 is associated with poor prognosis of ESCC patients. The present study thus demonstrates that phosphorylation of ETS-1 is a critical event in the Pol ι-induced invasion and metastasis of ESCC.

KW - DNA polymerase iota

KW - ETS-1 phosphorylation

KW - esophageal squamous cell carcinoma

KW - metastasis

KW - tumor invasion

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DO - 10.1111/cas.13399

M3 - Article

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AN - SCOPUS:85030629103

SN - 1347-9032

VL - 108

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JO - Cancer Science

JF - Cancer Science

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ER -

Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma

Abstract

Keywords

ASJC Scopus subject areas

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