TY - JOUR
T1 - Phorbol myristate acetate and dioctanoylglycerol inhibit transport in rabbit proximal convoluted tubule
AU - Baum, M.
AU - Hays, S. R.
PY - 1988
Y1 - 1988
N2 - The present in vitro microperfusion study examined the effect of protein kinase C activation on transport in the rabbit proximal convoluted tubule (PCT). PCT were perfused with an ultrafiltrate-like solution and were bathed in a serumlike albumin solution. Addition of 5 x 10-8 and 5 x 10-7 M bath phorbol 12-myristate 13-acetate, an activator of protein kinase C, inhibited volume absorption from 1.06 ± 0.10 to 0.77 ± 0.07 nl·mm-1·min-1 (P < 0.05) and 0.76 ± 0.14 to 0.48 ± 0.08 nl·mm-1·min-1 (P < 0.01), respectively. Bath phorbol 12-myristate 13-acetate (5 x 10-9 M) had no effect on volume absorption (J(v), 0.82 ± 0.13 to 0.81 ± 0.12 nl·mm-1·min-1). In contrast, 5 x 10-8 M bath 4α-phorbol, an inactive phorbol that does not activate protein kinase C, had no effect on J(v) (0.95 ± 0.14 to 0.94 ± 0.11 nl·mm-1·min-1). Bath L-α-dioctanoylglycerol (10-4 M), another known activator of protein kinase C, inhibited volume absorption from 0.96 ± 0.08 to 0.71 ± 0.08 nl·mm-1·min-1 (P < 0.001). A 10-fold lower concentration of L-α-dioctanoylglycerol (10-5 M) had no effect on J(v) (0.81 ± 0.18 to 0.78 ± 0.17 nl·mm-1·min-1). Both 5 x 10-8 M phorbol 12-myristate 13-acetate and 10-4 M L-α-dioctanoylglycerol inhibited glucose, bicarbonate, and chloride transport in the PCT. These data are consistent with protein kinase C activation playing a role in the modulation of proximal tubular transport.
AB - The present in vitro microperfusion study examined the effect of protein kinase C activation on transport in the rabbit proximal convoluted tubule (PCT). PCT were perfused with an ultrafiltrate-like solution and were bathed in a serumlike albumin solution. Addition of 5 x 10-8 and 5 x 10-7 M bath phorbol 12-myristate 13-acetate, an activator of protein kinase C, inhibited volume absorption from 1.06 ± 0.10 to 0.77 ± 0.07 nl·mm-1·min-1 (P < 0.05) and 0.76 ± 0.14 to 0.48 ± 0.08 nl·mm-1·min-1 (P < 0.01), respectively. Bath phorbol 12-myristate 13-acetate (5 x 10-9 M) had no effect on volume absorption (J(v), 0.82 ± 0.13 to 0.81 ± 0.12 nl·mm-1·min-1). In contrast, 5 x 10-8 M bath 4α-phorbol, an inactive phorbol that does not activate protein kinase C, had no effect on J(v) (0.95 ± 0.14 to 0.94 ± 0.11 nl·mm-1·min-1). Bath L-α-dioctanoylglycerol (10-4 M), another known activator of protein kinase C, inhibited volume absorption from 0.96 ± 0.08 to 0.71 ± 0.08 nl·mm-1·min-1 (P < 0.001). A 10-fold lower concentration of L-α-dioctanoylglycerol (10-5 M) had no effect on J(v) (0.81 ± 0.18 to 0.78 ± 0.17 nl·mm-1·min-1). Both 5 x 10-8 M phorbol 12-myristate 13-acetate and 10-4 M L-α-dioctanoylglycerol inhibited glucose, bicarbonate, and chloride transport in the PCT. These data are consistent with protein kinase C activation playing a role in the modulation of proximal tubular transport.
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U2 - 10.1152/ajprenal.1988.254.1.f9
DO - 10.1152/ajprenal.1988.254.1.f9
M3 - Article
C2 - 2827521
AN - SCOPUS:0023856212
SN - 0002-9513
VL - 254
SP - F9-F14
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
IS - 1 (23/1)
ER -