Peroxisomal protein import is conserved between yeast, plants, insects and mammals

Stephen J. Gould, Gilbert Andre Keller, Michel Schneider, Stephen H. Howell, Lisa J. Garrard, Joel M. Goodman, Ben Distel, Henk Tabak, Suresh Subramani

Research output: Contribution to journalArticlepeer-review

221 Scopus citations


We have previously demonstrated that firefly luciferase can be imported into peroxisomes of both insect and mammalian cells. To determine whether the process of protein transport into the peroxisome is functionally similar in more widely divergent eukaryotes, the cDNA encoding firefly luciferase was expressed in both yeast and plant cells. Luciferase was translocated into peroxisomes in each type of organism. Experiments were also performed to determine whether a yeast peroxisomal protein could be transported to peroxisomes in mammalian cells. We observed that a C-terminal segment of the yeast (candida boidinii) peroxisomal protein PMP20 could act as a peroxisomal targeting signal in mammalian cells. These results suggest that at least one mechanism of protein translocation into peroxisomes has been conserved throughout eukaryotic evolution.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalEMBO Journal
Issue number1
StatePublished - 1990


  • firefly luciferase
  • peroxisome
  • translocation
  • yeast

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


Dive into the research topics of 'Peroxisomal protein import is conserved between yeast, plants, insects and mammals'. Together they form a unique fingerprint.

Cite this