Peroxisomal protein import is conserved between yeast, plants, insects and mammals

Stephen J. Gould; Gilbert Andre Keller; Michel Schneider; Stephen H. Howell; Lisa J. Garrard; Joel M. Goodman; Ben Distel; Henk Tabak; Suresh Subramani

doi:10.1002/j.1460-2075.1990.tb08083.x

Peroxisomal protein import is conserved between yeast, plants, insects and mammals

Stephen J. Gould, Gilbert Andre Keller, Michel Schneider, Stephen H. Howell, Lisa J. Garrard, Joel M. Goodman, Ben Distel, Henk Tabak, Suresh Subramani

Research output: Contribution to journal › Article › peer-review

221 Scopus citations

Abstract

We have previously demonstrated that firefly luciferase can be imported into peroxisomes of both insect and mammalian cells. To determine whether the process of protein transport into the peroxisome is functionally similar in more widely divergent eukaryotes, the cDNA encoding firefly luciferase was expressed in both yeast and plant cells. Luciferase was translocated into peroxisomes in each type of organism. Experiments were also performed to determine whether a yeast peroxisomal protein could be transported to peroxisomes in mammalian cells. We observed that a C-terminal segment of the yeast (candida boidinii) peroxisomal protein PMP20 could act as a peroxisomal targeting signal in mammalian cells. These results suggest that at least one mechanism of protein translocation into peroxisomes has been conserved throughout eukaryotic evolution.

Original language	English (US)
Pages (from-to)	85-90
Number of pages	6
Journal	EMBO Journal
Volume	9
Issue number	1
DOIs	https://doi.org/10.1002/j.1460-2075.1990.tb08083.x
State	Published - 1990

Keywords

firefly luciferase
peroxisome
translocation
yeast

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1002/j.1460-2075.1990.tb08083.x

Cite this

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title = "Peroxisomal protein import is conserved between yeast, plants, insects and mammals",

abstract = "We have previously demonstrated that firefly luciferase can be imported into peroxisomes of both insect and mammalian cells. To determine whether the process of protein transport into the peroxisome is functionally similar in more widely divergent eukaryotes, the cDNA encoding firefly luciferase was expressed in both yeast and plant cells. Luciferase was translocated into peroxisomes in each type of organism. Experiments were also performed to determine whether a yeast peroxisomal protein could be transported to peroxisomes in mammalian cells. We observed that a C-terminal segment of the yeast (candida boidinii) peroxisomal protein PMP20 could act as a peroxisomal targeting signal in mammalian cells. These results suggest that at least one mechanism of protein translocation into peroxisomes has been conserved throughout eukaryotic evolution.",

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AU - Gould, Stephen J.

AU - Keller, Gilbert Andre

AU - Schneider, Michel

AU - Howell, Stephen H.

AU - Garrard, Lisa J.

AU - Goodman, Joel M.

AU - Distel, Ben

AU - Tabak, Henk

AU - Subramani, Suresh

PY - 1990

Y1 - 1990

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Peroxisomal protein import is conserved between yeast, plants, insects and mammals

Abstract

Keywords

ASJC Scopus subject areas

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