TY - JOUR
T1 - Peptidyl-prolyl isomerase 1 regulates Ca2+ handling by modulating sarco(Endo)plasmic reticulum calcium ATPase and Na2+/Ca2+ exchanger 1 protein levels and function
AU - Sacchi, Veronica
AU - Wang, Bingyan J.
AU - Kubli, Dieter
AU - Martinez, Alexander S.
AU - Jin, Jung Kang
AU - Alvarez, Roberto
AU - Hariharan, Nirmala
AU - Glembotski, Christopher
AU - Uchida, Takafumi
AU - Malter, James S.
AU - Yang, Yijun
AU - Gross, Polina
AU - Zhang, Chen
AU - Houser, Steven
AU - Rota, Marcello
AU - Sussman, Mark A.
N1 - Funding Information:
Sacchi was supported by Swiss National Found Fellowship (P2BSP3_155252). Wang is supported by the Rees Stealy Research Foundation Fellowship. Kubli is supported by NIH grant F32 HL136064-01. Jin was supported by the American Heart Association Postdoctoral Fellowship 16POST27510010. Malter is supported by NIH grants P01HL088594. Glembotski is supported by NIH grants R01 HL75573, R01 HL104535, R01 HL127439, and P01 HL085577. Sussman is supported by NIH grants R01HL067245, R37HL091102, R01HL105759, R01HL113647, R01HL117163, P01HL085577, and R01HL122525, as well as an award from the Fondation Leducq.
Publisher Copyright:
© 2017 The Authors.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Background--Aberrant Ca2+ handling is a prominent feature of heart failure. Elucidation of the molecular mechanisms responsible for aberrant Ca2+ handling is essential for the development of strategies to blunt pathological changes in calcium dynamics. The peptidyl-prolyl cis-trans isomerase peptidyl-prolyl isomerase 1 (Pin1) is a critical mediator of myocardial hypertrophy development and cardiac progenitor cell cycle. However, the influence of Pin1 on calcium cycling regulation has not been explored. On the basis of these findings, the aim of this study is to define Pin1 as a novel modulator of Ca2+ handling, with implications for improving myocardial contractility and potential for ameliorating development of heart failure. Methods and Results--Pin1 gene deletion or pharmacological inhibition delays cytosolic Ca2+ decay in isolated cardiomyocytes. Paradoxically, reduced Pin1 activity correlates with increased sarco(endo)plasmic reticulum calcium ATPase (SERCA2a) and Na2+/Ca2+ exchanger 1 protein levels. However, SERCA2a ATPase activity and calcium reuptake were reduced in sarcoplasmic reticulum membranes isolated from Pin1-deficient hearts, suggesting that Pin1 influences SERCA2a function. SERCA2a and Na2+/Ca2+ exchanger 1 associated with Pin1, as revealed by proximity ligation assay in myocardial tissue sections, indicating that regulation of Ca2+ handling within cardiomyocytes is likely influenced through Pin1 interaction with SERCA2a and Na2+/Ca2+ exchanger 1 proteins. Conclusions--Pin1 serves as a modulator of SERCA2a and Na2+/Ca2+ exchanger 1 Ca2+ handling proteins, with loss of function resulting in impaired cardiomyocyte relaxation, setting the stage for subsequent investigations to assess Pin1 dysregulation and modulation in the progression of heart failure.
AB - Background--Aberrant Ca2+ handling is a prominent feature of heart failure. Elucidation of the molecular mechanisms responsible for aberrant Ca2+ handling is essential for the development of strategies to blunt pathological changes in calcium dynamics. The peptidyl-prolyl cis-trans isomerase peptidyl-prolyl isomerase 1 (Pin1) is a critical mediator of myocardial hypertrophy development and cardiac progenitor cell cycle. However, the influence of Pin1 on calcium cycling regulation has not been explored. On the basis of these findings, the aim of this study is to define Pin1 as a novel modulator of Ca2+ handling, with implications for improving myocardial contractility and potential for ameliorating development of heart failure. Methods and Results--Pin1 gene deletion or pharmacological inhibition delays cytosolic Ca2+ decay in isolated cardiomyocytes. Paradoxically, reduced Pin1 activity correlates with increased sarco(endo)plasmic reticulum calcium ATPase (SERCA2a) and Na2+/Ca2+ exchanger 1 protein levels. However, SERCA2a ATPase activity and calcium reuptake were reduced in sarcoplasmic reticulum membranes isolated from Pin1-deficient hearts, suggesting that Pin1 influences SERCA2a function. SERCA2a and Na2+/Ca2+ exchanger 1 associated with Pin1, as revealed by proximity ligation assay in myocardial tissue sections, indicating that regulation of Ca2+ handling within cardiomyocytes is likely influenced through Pin1 interaction with SERCA2a and Na2+/Ca2+ exchanger 1 proteins. Conclusions--Pin1 serves as a modulator of SERCA2a and Na2+/Ca2+ exchanger 1 Ca2+ handling proteins, with loss of function resulting in impaired cardiomyocyte relaxation, setting the stage for subsequent investigations to assess Pin1 dysregulation and modulation in the progression of heart failure.
KW - Cardiomyocyte
KW - Na/Ca exchange
KW - Peptidyl-prolyl isomerase 1
KW - Sarcoplasmic reticulum Ca-ATPase
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U2 - 10.1161/JAHA.117.006837
DO - 10.1161/JAHA.117.006837
M3 - Article
C2 - 29018025
AN - SCOPUS:85032216047
SN - 2047-9980
VL - 6
JO - Journal of the American Heart Association
JF - Journal of the American Heart Association
IS - 10
M1 - e006837
ER -