Overexpression of Six1 leads to retardation of myogenic differentiation in C2C12 myoblasts

Zhixue Li, Daming Deng, Huocong Huang, Liang Tian, Zirong Chen, Youran Zou, Guorong Jin, Juan Wang, Qingjiong Zhang, Lizi Wu, Huangxuan Shen

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The Six1 homeoprotein belongs to the Six (sine oculis) transcription factor family, the members of which are known to act as master regulators of development. Six1 is essential for promoting myogenesis during mammalian somitogenesis. Previous studies have shown that Six1 participates in later steps of myogenic differentiation by enhancing early activation of myogenin via binding to the Mef3 site of the myogenin promoter. In the present study, however, we show that overexpression of Six1 via retroviral infection suppresses the expression of myogenin and myosin in C2C12 myoblasts, consequently retarding myogenic differentiation without affecting cell proliferation or expression of Mef2 and Mef3. These findings further demonstrate the functional role of Six1 in myogenesis.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalMolecular Biology Reports
Issue number1
StatePublished - Jan 2013
Externally publishedYes


  • Mef2
  • Mef3
  • Myogenic differentiation
  • Myogenin
  • Six1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Overexpression of Six1 leads to retardation of myogenic differentiation in C2C12 myoblasts'. Together they form a unique fingerprint.

Cite this