TY - JOUR
T1 - Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells
AU - Xia, Donglan
AU - Li, Xianting
AU - Lou, Yaxin
AU - Han, Wenling
AU - Ding, Peiguo
AU - Zhang, Yingmei
AU - Di, Chunhui
AU - Song, Quansheng
AU - Ma, Dalong
N1 - Funding Information:
This work was supported by grants from the National Natural Sciences Foundation of China (30000153) and under the auspices of the Chinese High Tech Program (863) and the National Key Project for Basic Research (G20000569). We thank Dr. Zhenguo Wu for the generous gifts of the reported plasmids, including the 4RE-Luc and 3×MEF2-Luc. We also thank Daniel Calvin for the critical reading of the manuscript.
PY - 2002/8/19
Y1 - 2002/8/19
N2 - Chemokine-like factor 1 (CKLF1) is a novel cytokine first cloned from U937 cells. It contains different splicing forms and has chemotactic effects on a wide spectrum of cells both in vitro and in vivo; it can also stimulate the regeneration of skeletal muscle cells in vivo, but the mechanism remains unclear. To probe the myogenesis function of CKLF2, which is the largest isoform of CKLFs, C2C12 murine myoblasts were stably transfected with human CKLF2 eukaryotic expression vector. Compared with control vector transfected C2C12 cells, CKLF2 overexpression causes accelerated myoblast proliferation as determined by cell counting and [3H]TdR incorporation assays. In addition, CKLF2 overexpression also promotes cell differentiation, which was determined by higher expression levels of myogenin, creatine kinase, myosin and the accelerated myoblast fusion. Further analysis also indicates that CKLF2 could activate the transcription activity of the bHLH/MyoD and MEF2 families. Finally, DNA synthesis and myotube formation could also be promoted by growing C2C12 cells in conditioned media from CKLF2-transfected cells. These findings strongly suggest a role for human CKLF2 in regulation of skeletal muscle myogenesis.
AB - Chemokine-like factor 1 (CKLF1) is a novel cytokine first cloned from U937 cells. It contains different splicing forms and has chemotactic effects on a wide spectrum of cells both in vitro and in vivo; it can also stimulate the regeneration of skeletal muscle cells in vivo, but the mechanism remains unclear. To probe the myogenesis function of CKLF2, which is the largest isoform of CKLFs, C2C12 murine myoblasts were stably transfected with human CKLF2 eukaryotic expression vector. Compared with control vector transfected C2C12 cells, CKLF2 overexpression causes accelerated myoblast proliferation as determined by cell counting and [3H]TdR incorporation assays. In addition, CKLF2 overexpression also promotes cell differentiation, which was determined by higher expression levels of myogenin, creatine kinase, myosin and the accelerated myoblast fusion. Further analysis also indicates that CKLF2 could activate the transcription activity of the bHLH/MyoD and MEF2 families. Finally, DNA synthesis and myotube formation could also be promoted by growing C2C12 cells in conditioned media from CKLF2-transfected cells. These findings strongly suggest a role for human CKLF2 in regulation of skeletal muscle myogenesis.
KW - C2C12
KW - Chemokine-like factor 2
KW - Differentiation
KW - Muscle regeneration
KW - Proliferation
KW - Skeletal muscle
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U2 - 10.1016/S0167-4889(02)00270-7
DO - 10.1016/S0167-4889(02)00270-7
M3 - Article
C2 - 12183067
AN - SCOPUS:0037135704
SN - 0167-4889
VL - 1591
SP - 163
EP - 173
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 1-3
ER -