TY - JOUR
T1 - Oracle, a novel PDZ-LIM domain protein expressed in heart and skeletal muscle
AU - Passier, Robert
AU - Richardson, James A.
AU - Olson, Eric N.
N1 - Funding Information:
We thank A. Tizenor for assistance with graphics and J. Starke and R. Wells for histologic preparations. This work was supported by grants from NIH to E.N.O. R.P. was supported by a fellowship from the Royal Netherlands Academy of Arts and Science.
PY - 2000/4/1
Y1 - 2000/4/1
N2 - In order to identify novel genes enriched in adult heart, we performed a subtractive hybridization for genes expressed in mouse heart but not in skeletal muscle. We identified two alternative splicing variants of a novel PDZ-LIM domain protein, which we named Oracle. Both variants contain a PDZ domain at the amino-terminus and three LIM domains at the carboxy-terminus. Highest homology of Oracle was found with the human and rat enigma proteins in the PDZ domain (62 and 61%, respectively) and in the LIM domains (60 and 69%, respectively). By Northern hybridization analysis, we showed that expression is highest in adult mouse heart, low in skeletal muscle and undetectable in other adult mouse tissues. In situ hybridization in mouse embryos confirmed and extended these data by showing high expression of Oracle mRNA in atrial and ventricular myocardial cells from E8.5. From E9.5 low expression of Oracle mRNA was detectable in myotomes. These data suggest a role for Oracle in the early development and function of heart and skeletal muscle. (C) 2000 Elsevier Science Ireland Ltd.
AB - In order to identify novel genes enriched in adult heart, we performed a subtractive hybridization for genes expressed in mouse heart but not in skeletal muscle. We identified two alternative splicing variants of a novel PDZ-LIM domain protein, which we named Oracle. Both variants contain a PDZ domain at the amino-terminus and three LIM domains at the carboxy-terminus. Highest homology of Oracle was found with the human and rat enigma proteins in the PDZ domain (62 and 61%, respectively) and in the LIM domains (60 and 69%, respectively). By Northern hybridization analysis, we showed that expression is highest in adult mouse heart, low in skeletal muscle and undetectable in other adult mouse tissues. In situ hybridization in mouse embryos confirmed and extended these data by showing high expression of Oracle mRNA in atrial and ventricular myocardial cells from E8.5. From E9.5 low expression of Oracle mRNA was detectable in myotomes. These data suggest a role for Oracle in the early development and function of heart and skeletal muscle. (C) 2000 Elsevier Science Ireland Ltd.
KW - Heart muscle
KW - PDZ-LIM domain protein
KW - Skeletal muscle
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U2 - 10.1016/S0925-4773(99)00330-5
DO - 10.1016/S0925-4773(99)00330-5
M3 - Article
C2 - 10727866
AN - SCOPUS:0034175815
SN - 2667-291X
VL - 92
SP - 277
EP - 284
JO - Cells and Development
JF - Cells and Development
IS - 2
ER -