Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene

P. J. Farnham; J. M. Abrams; R. T. Schimke

doi:10.1073/pnas.82.12.3978

Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene

P. J. Farnham, J. M. Abrams, R. T. Schimke

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Abstract

We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.

Original language	English (US)
Pages (from-to)	3978-3982
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	82
Issue number	12
DOIs	https://doi.org/10.1073/pnas.82.12.3978
State	Published - 1985

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abstract = "We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.",

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T1 - Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene

AU - Farnham, P. J.

AU - Abrams, J. M.

AU - Schimke, R. T.

PY - 1985

Y1 - 1985

N2 - We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.

AB - We have characterized a transcription unit in the 5' flanking region of the mouse gene encoding dihydrofolate reductase (EC 1.5.1.3) that is oriented in the opposite direction to that of dihydrofolate reductase transcription. These opposite-strand RNAs are 180-240 nucleotides long, differing in length at their 5' ends. They are abundant in nuclear RNA and are not polyadenylylated. We suggest that the promoter region of the dihydrofolate reductase gene functions in a bidirectional manner to produce both these RNAs and dihydrofolate reductase mRNAs.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene

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