TY - JOUR
T1 - Oligomeric structure of caveolin
T2 - Implications for caveolae membrane organization
AU - Sargiacomo, Massimo
AU - Scherer, Philipp E.
AU - Tang, ZhaoLan
AU - Kübler, Eric
AU - Song, Kenneth S.
AU - Sanders, Mitchell C.
AU - Lisanti, Michael P.
PY - 1995/9/26
Y1 - 1995/9/26
N2 - A 22-kDa protein, caveolin, is localized to the cytoplasmic surface of plasma membrane specializations called caveolae. We have proposed that caveolin may function as a scaffolding protein to organize and concentrate signaling molecules within caveolae. Here, we show that caveolin interacts with itself to form homooligomers. Electron microscopic visualization of these purified caveolin homooligomers demonstrates that they appear as individual spherical particles. By using recombinant expression of caveolin as a glutathione S-transferase fusion protein, we have defined a region of caveolin's cytoplasmic N-terminal domain that mediates these caveolin- caveolin interactions. We suggest that caveolin homooligomers may function to concentrate caveolin-interacting molecules within caveolae. In this regard, it may be useful to think of caveolin homooligomers as 'fishing lures' with multiple 'hooks' or attachment sites for caveolin-interacting molecules.
AB - A 22-kDa protein, caveolin, is localized to the cytoplasmic surface of plasma membrane specializations called caveolae. We have proposed that caveolin may function as a scaffolding protein to organize and concentrate signaling molecules within caveolae. Here, we show that caveolin interacts with itself to form homooligomers. Electron microscopic visualization of these purified caveolin homooligomers demonstrates that they appear as individual spherical particles. By using recombinant expression of caveolin as a glutathione S-transferase fusion protein, we have defined a region of caveolin's cytoplasmic N-terminal domain that mediates these caveolin- caveolin interactions. We suggest that caveolin homooligomers may function to concentrate caveolin-interacting molecules within caveolae. In this regard, it may be useful to think of caveolin homooligomers as 'fishing lures' with multiple 'hooks' or attachment sites for caveolin-interacting molecules.
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U2 - 10.1073/pnas.92.20.9407
DO - 10.1073/pnas.92.20.9407
M3 - Article
C2 - 7568142
AN - SCOPUS:0029075988
SN - 0027-8424
VL - 92
SP - 9407
EP - 9411
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -