TY - JOUR
T1 - Novel functional proteins coded by the human genome discovered in metastases of melanoma patients
AU - Sanchez, Aniel
AU - Kuras, Magdalena
AU - Murillo, Jimmy Rodriguez
AU - Pla, Indira
AU - Pawlowski, Krzysztof
AU - Szasz, A. Marcell
AU - Gil, Jeovanis
AU - Nogueira, Fábio C.S.
AU - Perez-Riverol, Yasset
AU - Eriksson, Jonatan
AU - Appelqvist, Roger
AU - Miliotis, Tasso
AU - Kim, Yonghyo
AU - Baldetorp, Bo
AU - Ingvar, Christian
AU - Olsson, Håkan
AU - Lundgren, Lotta
AU - Ekedahl, Henrik
AU - Horvatovich, Peter
AU - Sugihara, Yutaka
AU - Welinder, Charlotte
AU - Wieslander, Elisabet
AU - Kwon, Ho Jeong
AU - Domont, Gilberto B.
AU - Malm, Johan
AU - Rezeli, Melinda
AU - Betancourt, Lazaro Hiram
AU - Marko-Varga, György
N1 - Publisher Copyright:
© 2019, The Author(s).
PY - 2020/6/1
Y1 - 2020/6/1
N2 - In the advanced stages, malignant melanoma (MM) has a very poor prognosis. Due to tremendous efforts in cancer research over the last 10 years, and the introduction of novel therapies such as targeted therapies and immunomodulators, the rather dark horizon of the median survival has dramatically changed from under 1 year to several years. With the advent of proteomics, deep-mining studies can reach low-abundant expression levels. The complexity of the proteome, however, still surpasses the dynamic range capabilities of current analytical techniques. Consequently, many predicted protein products with potential biological functions have not yet been verified in experimental proteomic data. This category of ‘missing proteins’ (MP) is comprised of all proteins that have been predicted but are currently unverified. As part of the initiative launched in 2016 in the USA, the European Cancer Moonshot Center has performed numerous deep proteomics analyses on samples from MM patients. In this study, nine MPs were clearly identified by mass spectrometry in MM metastases. Some MPs significantly correlated with proteins that possess identical PFAM structural domains; and other MPs were significantly associated with cancer-related proteins. This is the first study to our knowledge, where unknown and novel proteins have been annotated in metastatic melanoma tumour tissue.
AB - In the advanced stages, malignant melanoma (MM) has a very poor prognosis. Due to tremendous efforts in cancer research over the last 10 years, and the introduction of novel therapies such as targeted therapies and immunomodulators, the rather dark horizon of the median survival has dramatically changed from under 1 year to several years. With the advent of proteomics, deep-mining studies can reach low-abundant expression levels. The complexity of the proteome, however, still surpasses the dynamic range capabilities of current analytical techniques. Consequently, many predicted protein products with potential biological functions have not yet been verified in experimental proteomic data. This category of ‘missing proteins’ (MP) is comprised of all proteins that have been predicted but are currently unverified. As part of the initiative launched in 2016 in the USA, the European Cancer Moonshot Center has performed numerous deep proteomics analyses on samples from MM patients. In this study, nine MPs were clearly identified by mass spectrometry in MM metastases. Some MPs significantly correlated with proteins that possess identical PFAM structural domains; and other MPs were significantly associated with cancer-related proteins. This is the first study to our knowledge, where unknown and novel proteins have been annotated in metastatic melanoma tumour tissue.
KW - Biobank
KW - Mass spectrometry
KW - Melanoma
KW - Missing proteins
KW - Proteomics
KW - Tissue
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U2 - 10.1007/s10565-019-09494-4
DO - 10.1007/s10565-019-09494-4
M3 - Article
C2 - 31599373
AN - SCOPUS:85074499088
SN - 0742-2091
VL - 36
SP - 261
EP - 272
JO - Cell Biology and Toxicology
JF - Cell Biology and Toxicology
IS - 3
ER -