Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

Brittney Shea Herbert; Amelia E. Hochreiter; Woodring E. Wright; Jerry W. Shay

doi:10.1038/nprot.2006.239

Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

Brittney Shea Herbert, Amelia E. Hochreiter, Woodring E. Wright, Jerry W. Shay

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198 Scopus citations

Abstract

The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).

Original language	English (US)
Pages (from-to)	1583-1590
Number of pages	8
Journal	Nature Protocols
Volume	1
Issue number	3
DOIs	https://doi.org/10.1038/nprot.2006.239
State	Published - Aug 2006

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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abstract = "The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).",

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note = "Funding Information: ACKNOWLEDGMENTS We thank P. Erickson and E. Badenhop for technical assistance. This work was supported by the Indiana University Cancer Center and the Indiana Genomics Initiative (INGEN). INGEN of Indiana University is supported in part by Lilly Endowment Inc.",

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Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

Abstract

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