Noninvasive investigation of blood oxygenation dynamics of tumors by near-infrared spectroscopy

Hanli Liu, Yulin Song, Katherine L. Worden, Xin Jiang, Anca Constantinescu, Ralph P. Mason

Research output: Contribution to journalArticlepeer-review

128 Scopus citations


The measurement of dynamic changes in the blood oxygenation of tumor vasculature could be valuable for tumor prognosis and optimizing tumor treatment plans. In this study we employed near-infrared spectroscopy (NIRS) to measure changes in the total hemoglobin concentration together with the degree of hemoglobin oxygenation in the vascular bed of breast and prostate tumors implanted in rats. Measurements were made while inhaled gas was alternated between 33% oxygen and carbogen (95% O2, 5% CO2). Significant dynamic changes in tumor oxygenation were observed to accompany respiratory challenge, and these changes could be modeled with two exponential components, yielding two time constants. Following the Fick principle, we derived a simplified model to relate the time constants to tumor blood-perfusion rates. This study demonstrates that the NIRS technology can provide an efficient, real-time, noninvasive means of monitoring the vascular oxygenation dynamics of tumors and facilitate investigations of tumor vascular perfusion. This may have prognostic value and promises insight into tumor vascular development.

Original languageEnglish (US)
Pages (from-to)5231-5243
Number of pages13
JournalApplied Optics
Issue number28
StatePublished - Oct 1 2000

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering


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