TY - JOUR
T1 - New views of cells in 3D
T2 - An introduction to electron tomography
AU - McIntosh, Richard
AU - Nicastro, Daniela
AU - Mastronarde, David
N1 - Funding Information:
We thank E. O'Toole and A. Staehelin for criticism of the manuscript; K. Grunewald, M. Martone, M. Moritz, S. Nickell and M. Otegui for use of their micrographs; K. Christensen (Imagecyte) for providing bacteriophage cartoons; and the National Institutes of Health (GM 61306) and the National Center for Research Resources (RR 00592) for financial support.
PY - 2005/1
Y1 - 2005/1
N2 - The most important goal of structural cell biology is to elucidate the mechanisms of the processes of life. The structure of a membrane system or fibrous array, the changes in such structures over time or the localizations of enzymes relative to organelle boundaries can often illuminate associated cellular functions. To be of maximal value, structural studies should provide isotropic, 3D information about well-preserved samples at the highest possible resolution. Electron tomography can provide such information about many kinds of cells and organelles.
AB - The most important goal of structural cell biology is to elucidate the mechanisms of the processes of life. The structure of a membrane system or fibrous array, the changes in such structures over time or the localizations of enzymes relative to organelle boundaries can often illuminate associated cellular functions. To be of maximal value, structural studies should provide isotropic, 3D information about well-preserved samples at the highest possible resolution. Electron tomography can provide such information about many kinds of cells and organelles.
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U2 - 10.1016/j.tcb.2004.11.009
DO - 10.1016/j.tcb.2004.11.009
M3 - Review article
C2 - 15653077
AN - SCOPUS:11844305068
SN - 0962-8924
VL - 15
SP - 43
EP - 51
JO - Trends in Cell Biology
JF - Trends in Cell Biology
IS - 1
ER -