Abstract
The ligand, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylenephosphonic acid, ethyl ester) (DOTPME) was made membrane permeable by preparing its acetoxymethyl (AM) derivative (DOTPME-AM). The synthetic approach was to prepare the AM ester of the phosphonate side-chain prior to attachment to the macrocyclic ring. 31P NMR was used to demonstrate that DOTPME-AM can penetrate cell membranes, get hydrolyzed by cellular esterases to regenerate charged DOTPME, and hence become trapped inside cells. This technology offers the potential of designing Ca 2+ and Mg 2+ specific ligands for analytical, noninvasive measurement of these ions by 31P NMR.
Original language | English (US) |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Publisher | Society of Photo-Optical Instrumentation Engineers |
Pages | 99-106 |
Number of pages | 8 |
Volume | 3600 |
State | Published - 1999 |
Event | Proceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation - San Jose, CA, USA Duration: Jan 26 1999 → Jan 28 1999 |
Other
Other | Proceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation |
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City | San Jose, CA, USA |
Period | 1/26/99 → 1/28/99 |
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics