Near-infrared fluorescence imaging of carotid plaques in an atherosclerotic murine model

Xiaotian Wu; Amy Daniel Ulumben; Steven Long; Wataru Katagiri; Moses Q. Wilks; Hushan Yuan; Brian Cortese; Chengeng Yang; Satoshi Kashiwagi; Hak Soo Choi; Marc D. Normandin; Georges El Fakhri; Raiyan T. Zaman

doi:10.3390/biom11121753

Near-infrared fluorescence imaging of carotid plaques in an atherosclerotic murine model

Xiaotian Wu, Amy Daniel Ulumben, Steven Long, Wataru Katagiri, Moses Q. Wilks, Hushan Yuan, Brian Cortese, Chengeng Yang, Satoshi Kashiwagi, Hak Soo Choi, Marc D. Normandin, Georges El Fakhri, Raiyan T. Zaman

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Successful imaging of atherosclerosis, one of the leading global causes of death, is crucial for diagnosis and intervention. Near-infrared fluorescence (NIRF) imaging has been widely adopted along with multimodal/hybrid imaging systems for plaque detection. We evaluate two macrophage-targeting fluorescent tracers for NIRF imaging (TLR4-ZW800-1C and Feraheme-Alexa Fluor 750) in an atherosclerotic murine cohort, where the left carotid artery (LCA) is ligated to cause stenosis, and the right carotid artery (RCA) is used as a control. Imaging performed on dissected tissues revealed that both tracers had high uptake in the diseased vessel compared to the control, which was readily visible even at short exposure times. In addition, ZW800-1C’s renal clearance ability and Feraheme’s FDA approval puts these two tracers in line with other NIRF tracers such as ICG. Continued investigation with these tracers using intravascular NIRF imaging and larger animal models is warranted for clinical translation.

Original language	English (US)
Article number	1753
Journal	Biomolecules
Volume	11
Issue number	12
DOIs	https://doi.org/10.3390/biom11121753
State	Published - Dec 2021
Externally published	Yes

Keywords

Atherosclerosis
Feraheme
Macrophages
Molecular-targeted fluorescent tracers
NIRF
TLR4
Zwitterionic

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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title = "Near-infrared fluorescence imaging of carotid plaques in an atherosclerotic murine model",

abstract = "Successful imaging of atherosclerosis, one of the leading global causes of death, is crucial for diagnosis and intervention. Near-infrared fluorescence (NIRF) imaging has been widely adopted along with multimodal/hybrid imaging systems for plaque detection. We evaluate two macrophage-targeting fluorescent tracers for NIRF imaging (TLR4-ZW800-1C and Feraheme-Alexa Fluor 750) in an atherosclerotic murine cohort, where the left carotid artery (LCA) is ligated to cause stenosis, and the right carotid artery (RCA) is used as a control. Imaging performed on dissected tissues revealed that both tracers had high uptake in the diseased vessel compared to the control, which was readily visible even at short exposure times. In addition, ZW800-1C{\textquoteright}s renal clearance ability and Feraheme{\textquoteright}s FDA approval puts these two tracers in line with other NIRF tracers such as ICG. Continued investigation with these tracers using intravascular NIRF imaging and larger animal models is warranted for clinical translation.",

keywords = "Atherosclerosis, Feraheme, Macrophages, Molecular-targeted fluorescent tracers, NIRF, TLR4, Zwitterionic",

author = "Xiaotian Wu and {Daniel Ulumben}, Amy and Steven Long and Wataru Katagiri and Wilks, {Moses Q.} and Hushan Yuan and Brian Cortese and Chengeng Yang and Satoshi Kashiwagi and Choi, {Hak Soo} and Normandin, {Marc D.} and {El Fakhri}, Georges and Zaman, {Raiyan T.}",

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year = "2021",

month = dec,

doi = "10.3390/biom11121753",

language = "English (US)",

volume = "11",

journal = "Biomolecules",

issn = "2218-273X",

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T1 - Near-infrared fluorescence imaging of carotid plaques in an atherosclerotic murine model

AU - Wu, Xiaotian

AU - Daniel Ulumben, Amy

AU - Long, Steven

AU - Katagiri, Wataru

AU - Wilks, Moses Q.

AU - Yuan, Hushan

AU - Cortese, Brian

AU - Yang, Chengeng

AU - Kashiwagi, Satoshi

AU - Choi, Hak Soo

AU - Normandin, Marc D.

AU - El Fakhri, Georges

AU - Zaman, Raiyan T.

PY - 2021/12

Y1 - 2021/12

N2 - Successful imaging of atherosclerosis, one of the leading global causes of death, is crucial for diagnosis and intervention. Near-infrared fluorescence (NIRF) imaging has been widely adopted along with multimodal/hybrid imaging systems for plaque detection. We evaluate two macrophage-targeting fluorescent tracers for NIRF imaging (TLR4-ZW800-1C and Feraheme-Alexa Fluor 750) in an atherosclerotic murine cohort, where the left carotid artery (LCA) is ligated to cause stenosis, and the right carotid artery (RCA) is used as a control. Imaging performed on dissected tissues revealed that both tracers had high uptake in the diseased vessel compared to the control, which was readily visible even at short exposure times. In addition, ZW800-1C’s renal clearance ability and Feraheme’s FDA approval puts these two tracers in line with other NIRF tracers such as ICG. Continued investigation with these tracers using intravascular NIRF imaging and larger animal models is warranted for clinical translation.

AB - Successful imaging of atherosclerosis, one of the leading global causes of death, is crucial for diagnosis and intervention. Near-infrared fluorescence (NIRF) imaging has been widely adopted along with multimodal/hybrid imaging systems for plaque detection. We evaluate two macrophage-targeting fluorescent tracers for NIRF imaging (TLR4-ZW800-1C and Feraheme-Alexa Fluor 750) in an atherosclerotic murine cohort, where the left carotid artery (LCA) is ligated to cause stenosis, and the right carotid artery (RCA) is used as a control. Imaging performed on dissected tissues revealed that both tracers had high uptake in the diseased vessel compared to the control, which was readily visible even at short exposure times. In addition, ZW800-1C’s renal clearance ability and Feraheme’s FDA approval puts these two tracers in line with other NIRF tracers such as ICG. Continued investigation with these tracers using intravascular NIRF imaging and larger animal models is warranted for clinical translation.

KW - Atherosclerosis

KW - Feraheme

KW - Macrophages

KW - Molecular-targeted fluorescent tracers

KW - NIRF

KW - TLR4

KW - Zwitterionic

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ER -

Near-infrared fluorescence imaging of carotid plaques in an atherosclerotic murine model

Abstract

Keywords

ASJC Scopus subject areas

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