@article{44a7a57e3028437eab0b080b59cd5ec2,
title = " NCR − group 3 innate lymphoid cells orchestrate IL-23/IL-17 axis to promote hepatocellular carcinoma development ",
abstract = " Background: Innate lymphoid cells (ILCs) are a newly discovered family of immune cells that have similar cytokine-secreting profiles as T helper cell subsets. Although ILCs are critical for host defense against infections and tissue homeostasis, their roles in tumor development are not well established. Methods: We studied the function of ILC3 cells in the liver for the development of hepatocellular carcinoma (HCC) in murine HCC models using flow cytometry, adoptive transfer, and in vitro functional assays. Findings: We found that ILC3 lacking the natural cytotoxicity-triggering receptor (NCR − ILC3) promoted the development of HCC in response to interleukin 23 (IL-23). IL-23 serum level is elevated in HCC patients and its high expression is associated with poor clinical outcomes. We found that IL-23 could promote tumor development in murine HCC tumor models. IL-23 promoted the expansion of NCR − ILC3 and its differentiation from group 1 ILCs (ILC1s). Furthermore, NCR − ILC3 initiated IL-17 production upon IL-23 stimulation and directly inhibited CD8 + T cell immunity by promoting lymphocyte apoptosis and limiting their proliferation. Interpretation: Together, our findings suggest that NCR − ILC3 initiates the IL-17-rich immunosuppressive tumor microenvironment and promotes the development of HCC, thus may serve as a promising target for future cancer immunotherapy. Fund: This work was supported by grants from National Natural Science Foundation of China (81471586, 81571556), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the collaborative Innovation Center of Hematology, start-up grant from National University of Singapore, the Cancer Prevention and Research Institute of Texas CPRIT (RR180017), and the National Cancer Institute's Cancer Center Support (Core) Grant CA016672 (to The University of Texas MD Anderson Cancer Center).",
keywords = "HCC, IL-17, IL-23, ILC, Tumor microenvironment",
author = "Yonghao Liu and Yuan Song and Dandan Lin and Lei Lei and Yu Mei and Ziqi Jin and Huanle Gong and Ying Zhu and Bo Hu and Yinsheng Zhang and Lixiang Zhao and Teo, {Huey Yee} and Ju Qiu and Wen Jiang and Chen Dong and Depei Wu and Yuhui Huang and Haiyan Liu",
note = "Funding Information: This work was supported by grants from National Natural Science Foundation of China (81471586, 81571556), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Collaborative Innovation Center of Hematology, a start-up grant from the National University of Singapore, the Cancer Prevention and Research Institute of Texas CPRIT (PR180017), and the National Cancer Institute's Cancer Center Support (Core) Grant CA016672 (to The University of Texas MD Anderson Cancer Center). All funding sources have no role in study design, in the collection, analysis, and interpretation of data, in the writing of the manuscript, and in the decision to submit the paper for publication. Funding Information: This work was supported by grants from National Natural Science Foundation of China ( 81471586 , 81571556 ), the Priority Academic Program Development of Jiangsu Higher Education Institutions , the Collaborative Innovation Center of Hematology , a start-up grant from the National University of Singapore , the Cancer Prevention and Research Institute of Texas CPRIT ( PR180017 ), and the National Cancer Institute {\textquoteleft}s Cancer Center Support (Core) Grant CA016672 (to The University of Texas MD Anderson Cancer Center ). All funding sources have no role in study design, in the collection, analysis, and interpretation of data, in the writing of the manuscript, and in the decision to submit the paper for publication. Funding Information: We thank Dr. Yiqiang Wang, Dr. Yun Zhao and Dr. Jianhong Chu of Soochow University for helpful discussions. The authors also thank C. Wogan of MD Anderson Cancer Center's Division of Radiation Oncology for editorial assistance. This work was supported by grants from National Natural Science Foundation of China (81471586, 81571556), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Collaborative Innovation Center of Hematology, a start-up grant from the National University of Singapore, the Cancer Prevention and Research Institute of Texas CPRIT (PR180017), and the National Cancer Institute?s Cancer Center Support (Core) Grant CA016672 (to The University of Texas MD Anderson Cancer Center). All funding sources have no role in study design, in the collection, analysis, and interpretation of data, in the writing of the manuscript, and in the decision to submit the paper for publication. The authors declare no competing financial interests. YL and HL designed the experiments; YL, YS, DL, LL, YM, ZJ, HG, YZ, BH, YZ, LZ, and HYT performed the experiments; YL, YS, WJ, DW, YH, and HL analyzed data and interpreted results; YL, YS, YH and HL wrote the manuscript, JQ and CD provided critical experimental materials, all authors commented on the manuscript. Publisher Copyright: {\textcopyright} 2019",
year = "2019",
month = mar,
doi = "10.1016/j.ebiom.2019.02.050",
language = "English (US)",
volume = "41",
pages = "333--344",
journal = "EBioMedicine",
issn = "2352-3964",
publisher = "Elsevier BV",
}