Nanoparticulate cellular patches for cell-mediated tumoritropic delivery

Hao Cheng; Christian J. Kastrup; Renuka Ramanathan; Daniel J. Siegwart; Minglin Ma; Said R. Bogatyrev; Qiaobing Xu; Kathryn A. Whitehead; Robert Langer; Daniel G. Anderson

doi:10.1021/nn901319y

Nanoparticulate cellular patches for cell-mediated tumoritropic delivery

Hao Cheng, Christian J. Kastrup, Renuka Ramanathan, Daniel J. Siegwart, Minglin Ma, Said R. Bogatyrev, Qiaobing Xu, Kathryn A. Whitehead, Robert Langer, Daniel G. Anderson

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. NeutrAvidin-coated nanoparticles were anchored on cells possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix. Additionally, human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.

Original language	English (US)
Pages (from-to)	625-631
Number of pages	7
Journal	ACS Nano
Volume	4
Issue number	2
DOIs	https://doi.org/10.1021/nn901319y
State	Published - Feb 23 2010

Keywords

Cell therapy
Drug delivery
Endocytosis
Lipid raft
Membrane curvature
Tissue Engineering

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

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10.1021/nn901319y

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title = "Nanoparticulate cellular patches for cell-mediated tumoritropic delivery",

abstract = "The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. NeutrAvidin-coated nanoparticles were anchored on cells possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix. Additionally, human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.",

keywords = "Cell therapy, Drug delivery, Endocytosis, Lipid raft, Membrane curvature, Tissue Engineering",

author = "Hao Cheng and Kastrup, {Christian J.} and Renuka Ramanathan and Siegwart, {Daniel J.} and Minglin Ma and Bogatyrev, {Said R.} and Qiaobing Xu and Whitehead, {Kathryn A.} and Robert Langer and Anderson, {Daniel G.}",

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AU - Cheng, Hao

AU - Kastrup, Christian J.

AU - Ramanathan, Renuka

AU - Siegwart, Daniel J.

AU - Ma, Minglin

AU - Bogatyrev, Said R.

AU - Xu, Qiaobing

AU - Whitehead, Kathryn A.

AU - Langer, Robert

AU - Anderson, Daniel G.

PY - 2010/2/23

Y1 - 2010/2/23

N2 - The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. NeutrAvidin-coated nanoparticles were anchored on cells possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix. Additionally, human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.

AB - The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. NeutrAvidin-coated nanoparticles were anchored on cells possessing biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix. Additionally, human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.

KW - Cell therapy

KW - Drug delivery

KW - Endocytosis

KW - Lipid raft

KW - Membrane curvature

KW - Tissue Engineering

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Nanoparticulate cellular patches for cell-mediated tumoritropic delivery

Abstract

Keywords

ASJC Scopus subject areas

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