NADPH-dependent microsomal metabolism of 14,15-epoxyeicosatrienoic acid to diepoxides and epoxyalcohols

Jorge H. Capdevila, Paul Mosset, Pendri Yadagiri, Sun Lumin, J. R. Falck

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


The arachidonic acid epoxygenase metabolite 14,15-epoxyeicosatrienoic acid is further metabolized by rat liver microsomal fractions to regioisomeric diepoxides and epoxyalcohols. Diepoxides result from epoxidation at the 5,6-, 8,9-, or 11,12-olefins. Hydroxylation leading to epoxyalcohols with a cis, trans-conjugated dienol occurs at carbons 5, 8, 9, or 12. Structural assignments were established by chromatographic and mass spectral comparisons with synthetic standards. The reaction requires NADPH and is inhibited by typical cytochrome P-450 inhibitors. Analysis of the time course of product formation during arachidonic acid oxidation by rat liver microsomal fractions indicated that all four regioisomeric epoxyeicosatrienoic acids can be further metabolized by the enzyme system.

Original languageEnglish (US)
Pages (from-to)122-133
Number of pages12
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Feb 15 1988

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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