@article{eff79513b0be4e16baf2eb39e0b9de59,
title = "Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling",
abstract = "The SNF2 family ATPase Amplified in Liver Cancer 1 (ALC1) is the only chromatin remodeling enzyme with a poly(ADP-ribose) (PAR) binding macrodomain. ALC1 functions together with poly(ADPribose) polymerase PARP1 to remodel nucleosomes. Activation of ALC1 cryptic ATPase activity and the subsequent nucleosome remodeling requires binding of its macrodomain to PAR chains synthesized by PARP1 and NAD+. A key question is whether PARP1 has a role(s) in ALC1-dependent nucleosome remodeling beyond simply synthesizing the PAR chains needed to activate the ALC1 ATPase. Here, we identify PARP1 separation-of-function mutants that activate ALC1 ATPase but do not support nucleosome remodeling by ALC1. Investigation of these mutants has revealed multiple functions for PARP1 in ALC1-dependent nucleosome remodeling and provides insights into its multifaceted role in chromatin remodeling.",
keywords = "CHD1L, Nucleosome binding, Nucleosome remodeling, Poly(ADP-ribose) synthesis, SNF2 family ATPase",
author = "Ooi, {Soon Keat} and Shigeo Sato and Chieri Tomomori-Sato and Ying Zhang and Zhihui Wen and Banks, {Charles A.S.} and Washburn, {Michael P.} and Unruh, {Jay R.} and Laurence Florens and Conaway, {Ronald C.} and Conaway, {Joan W.}",
note = "Funding Information: ACKNOWLEDGMENTS. We thank John Pascal and Marie-France Langelier for advice about PARP1 expression and purification and for a gift of an expression plasmid encoding wild-type PARP1. We are also grateful to Karolin Luger, Uma Muthurajan, and Maggie Chasse for the gift of purified PARP1, ΔZn2, and ΔZn2ΔBRCT mutants used in the initial phase of this study. We also thank Merry McLaird and Michaella Levy for help with PARP1 expression and purification and Aaron J. Gottschalk, Rushi Trivedi, and Boris Rubenstein for helpful discussions. This work was supported by the Stowers Institute for Medical Research and by a grant to the Stowers Institute from the Helen Nelson Medical Research Fund at the Greater Kansas City Community Foundation. Publisher Copyright: {\textcopyright} 2021 National Academy of Sciences. All rights reserved.",
year = "2021",
month = sep,
day = "7",
doi = "10.1073/pnas.2107277118",
language = "English (US)",
volume = "118",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "36",
}