TY - JOUR
T1 - Morphology of a 'noninfectious' sarcoma virus
AU - Hall, W. T.
AU - Gazdar, A. F.
AU - Hobbs, B. A.
AU - Chopra, H. C.
N1 - Funding Information:
1 Received November 13, 1973; accepted January 16, 1974. 2 Electro-Nucleonics Laboratories, Inc., 4905 Del Ray Ave., Bethesda, Md. 20014. 3 Viral Leukemia and Lymphoma Branch, National Cancer Institute, National Institutes of Health, Public Health Service, V.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. 4 Viral Biology Branch, National Cancer Institute.
PY - 1974
Y1 - 1974
N2 - Two virus producing cell culture lines, HTG2 and HTG3, were established from a transplantable hamster tumor induced by a murine sarcoma virus (MSV) after 17 and 60 in vivo passages, respectively. HTG2 cells presumably produced only noninfectious sarcoma virions, whereas HTG3 cells yielded both sarcoma and 'helper' viruses. Electron microscopic examination of the original virus induced tumors, of pellets from cells grown in tissue culture, and of tissue culture medium harvested at 24, 48, 72, and 96 hr consistently revealed the same morphologic characteristics: More than 99% of the HTG2 virions retained their electron lucent or 'immature' form, in marked contrast to HTG3 virions or other typical type C viruses, which formed electron dense nucleoids. In addition, a large percentage of both HTG2 and HTG3 particles appeared morphologically incomplete, though completely detached or released from the cells. The inability to form dense nucleoids may be characteristic of noninfectious murine sarcoma viruses.
AB - Two virus producing cell culture lines, HTG2 and HTG3, were established from a transplantable hamster tumor induced by a murine sarcoma virus (MSV) after 17 and 60 in vivo passages, respectively. HTG2 cells presumably produced only noninfectious sarcoma virions, whereas HTG3 cells yielded both sarcoma and 'helper' viruses. Electron microscopic examination of the original virus induced tumors, of pellets from cells grown in tissue culture, and of tissue culture medium harvested at 24, 48, 72, and 96 hr consistently revealed the same morphologic characteristics: More than 99% of the HTG2 virions retained their electron lucent or 'immature' form, in marked contrast to HTG3 virions or other typical type C viruses, which formed electron dense nucleoids. In addition, a large percentage of both HTG2 and HTG3 particles appeared morphologically incomplete, though completely detached or released from the cells. The inability to form dense nucleoids may be characteristic of noninfectious murine sarcoma viruses.
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U2 - 10.1093/jnci/52.4.1337
DO - 10.1093/jnci/52.4.1337
M3 - Article
C2 - 4363711
AN - SCOPUS:0016364397
SN - 0027-8874
VL - 52
SP - 1337
EP - 1343
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 4
ER -