Monomolecular multimodal fluorescence-radioisotope imaging agents

Zongren Zhang, Kexian Liang, Sharon Bloch, Mikhail Berezin, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review

57 Scopus citations


Diagnosis of diseases by different imaging methods can provide complementary information about the functional status of diseased tissues or organs. To overcome the current difficulties in coregistering images from different imaging modalities with a high degree of accuracy, we prepared near-infrared (NIR) monomolecular multimodal imaging agents (MOMIAs) consisting of a heptamethine carbocyanine and 111In-DOTA chelate that served as antennae for optical and scintigraphic imaging, respectively. Their spectral properties clearly show that coordination of indium to MOMIA increased the fluorescence intensity of the compounds. The MOMIAs are exceptionally stable in biological media and serum up to 24 h at 37 °C. Biodistribution of the compounds in mice obtained by fluorescence photon and γ-counts demonstrated a similar distribution trend of the molecular probe in different tissues, suggesting that the detected fluorescence and γ-emissions emanated from the same source (MOMIA). At 24 h postinjection, the MOMIAs were excreted by the renal and hepatobiliary systems and the blood level of a representative MOMIA was very low, thereby reducing background noise caused by circulating molecular probes. These findings demonstrate the feasibility of preparing single molecules with the capacity to emit discernible and diagnostic fluorescent and γ-radiations for optical and nuclear imaging of living organisms.

Original languageEnglish (US)
Pages (from-to)1232-1239
Number of pages8
JournalBioconjugate Chemistry
Issue number5
StatePublished - 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry


Dive into the research topics of 'Monomolecular multimodal fluorescence-radioisotope imaging agents'. Together they form a unique fingerprint.

Cite this