Monoclonal antibodies to endothelin: Application for sandwich immunoassays

M. Hamaoki, H. Kato, M. Sugi, M. Fujimoto, H. Kurihara, M. Yosizumi, M. Yanagisawa, S. Kimura, T. Masaki, Y. Yazaki

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
Issue number1
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology
  • Genetics


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