@inbook{f6bddb9c54644fcf85dbf1dda0e5204b,
title = "Monitoring wnt protein acylation using an in vitro cyclo- addition reaction",
abstract = "We describe here a technique for visualizing the lipidation status of Wnt proteins using azide-alkyne cycloaddition chemistry (click chemistry) and SDS-PAGE. This protocol incorporates in vivo labeling of a Wnt- IgG Fc fusion protein using an alkynylated palmitate probe but departs from a traditional approach by incorporating a secondary cycloaddition reaction performed on single-step purified Wnt protein immobilized on protein A resin. This approach mitigates experimental noise by decreasing the contribution of labeling from other palmitoylated proteins and by providing a robust method for normalizing labeling efficiency based on protein abundance.",
keywords = "Biotinylation, Click chemistry, IgG fusion protein, Palmitoleate, Wnt acylation",
author = "Rubina Tuladhar and Nageswari Yarravarapu and Lawrence Lum",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-6393-5_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "11--16",
booktitle = "Methods in Molecular Biology",
}