TY - CHAP
T1 - Monitoring autophagy-dependent ferroptosis
AU - Li, Jingbo
AU - Kang, Rui
AU - Tang, Daolin
N1 - Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/1
Y1 - 2021/1
N2 - Ferroptosis is an iron-dependent form of regulated cell death, driven by the accumulation of lipid peroxidation. Autophagy is a lysosome-dependent degradation process that can be used to remove and recover intracellular components, such as dysfunctional proteins and damaged organelles. By regulating iron storage and oxidative stress, excessive autophagy is involved in the induction and execution of ferroptosis. In particular, several types of selective autophagy (e.g., ferritinophagy, lipophagy, clockophagy, and chaperone-mediated autophagy) increase the susceptibility to ferroptotic cell death by degrading anti-ferroptotic regulators (e.g., ferritin, GPX4, ARNTL, and lipid droplets). These two integrated biological processes play a pathological role in the occurrence and development of human diseases, such as cancer, neurodegenerative disorders, ischemia and reperfusion injury. Therefore, it is important to develop reliable methods to evaluate the kinetics of autophagosome formation, iron accumulation, and lipid peroxidation. Here, we introduce some protocols (such as western blotting, lipid peroxidation assay kits and probes, and iron probes) to monitor the process of autophagy-dependent ferroptosis.
AB - Ferroptosis is an iron-dependent form of regulated cell death, driven by the accumulation of lipid peroxidation. Autophagy is a lysosome-dependent degradation process that can be used to remove and recover intracellular components, such as dysfunctional proteins and damaged organelles. By regulating iron storage and oxidative stress, excessive autophagy is involved in the induction and execution of ferroptosis. In particular, several types of selective autophagy (e.g., ferritinophagy, lipophagy, clockophagy, and chaperone-mediated autophagy) increase the susceptibility to ferroptotic cell death by degrading anti-ferroptotic regulators (e.g., ferritin, GPX4, ARNTL, and lipid droplets). These two integrated biological processes play a pathological role in the occurrence and development of human diseases, such as cancer, neurodegenerative disorders, ischemia and reperfusion injury. Therefore, it is important to develop reliable methods to evaluate the kinetics of autophagosome formation, iron accumulation, and lipid peroxidation. Here, we introduce some protocols (such as western blotting, lipid peroxidation assay kits and probes, and iron probes) to monitor the process of autophagy-dependent ferroptosis.
KW - Autophagy
KW - Cell death
KW - Ferroptosis
KW - Lipid peroxidation
KW - Metabolism
UR - http://www.scopus.com/inward/record.url?scp=85096189842&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85096189842&partnerID=8YFLogxK
U2 - 10.1016/bs.mcb.2020.10.012
DO - 10.1016/bs.mcb.2020.10.012
M3 - Chapter
C2 - 34311865
AN - SCOPUS:85096189842
SN - 9780128244876
T3 - Methods in Cell Biology
SP - 163
EP - 176
BT - Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B
A2 - Kepp, Oliver
A2 - Galluzzi, Lorenzo
PB - Academic Press Inc.
ER -