Molecular weight determination of nucleic acids and proteins

Ravi Prakash; Pramod Kumar Singh; Santosh Kumar Jatav; Ajay Kumar Gautam

doi:10.1016/B978-0-12-822654-4.00009-9

Molecular weight determination of nucleic acids and proteins

Ravi Prakash, Pramod Kumar Singh, Santosh Kumar Jatav, Ajay Kumar Gautam

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The determination of the molecular weight of nucleic acids and proteins has found wide application in biosciences especially as it relates to the evaluation of molecular properties, separation, identification, purification, and characterization of nucleic acids and proteins. The commonly employed techniques for the determination of molecular weights (MWs) include electrophoresis, centrifugation, precipitation, chromatography, and mass spectrometry. In this chapter, authors presented in detail the MW determination of nucleic acids and proteins and the techniques employed for successful determinations.

Original language	English (US)
Title of host publication	Analytical Techniques in Biosciences
Subtitle of host publication	from Basics to Applications
Publisher	Elsevier
Pages	147-156
Number of pages	10
ISBN (Electronic)	9780128226544
ISBN (Print)	9780128227992
DOIs	https://doi.org/10.1016/B978-0-12-822654-4.00009-9
State	Published - Jan 1 2022
Externally published	Yes

Keywords

DNA
Protein
RNA molecular weight
chromatography

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/B978-0-12-822654-4.00009-9

Cite this

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AU - Gautam, Ajay Kumar

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Y1 - 2022/1/1

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AB - The determination of the molecular weight of nucleic acids and proteins has found wide application in biosciences especially as it relates to the evaluation of molecular properties, separation, identification, purification, and characterization of nucleic acids and proteins. The commonly employed techniques for the determination of molecular weights (MWs) include electrophoresis, centrifugation, precipitation, chromatography, and mass spectrometry. In this chapter, authors presented in detail the MW determination of nucleic acids and proteins and the techniques employed for successful determinations.

KW - DNA

KW - Protein

KW - RNA molecular weight

KW - chromatography

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SN - 9780128227992

SP - 147

EP - 156

BT - Analytical Techniques in Biosciences

PB - Elsevier

ER -

Molecular weight determination of nucleic acids and proteins

Abstract

Keywords

ASJC Scopus subject areas

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