Abstract
The determination of the molecular weight of nucleic acids and proteins has found wide application in biosciences especially as it relates to the evaluation of molecular properties, separation, identification, purification, and characterization of nucleic acids and proteins. The commonly employed techniques for the determination of molecular weights (MWs) include electrophoresis, centrifugation, precipitation, chromatography, and mass spectrometry. In this chapter, authors presented in detail the MW determination of nucleic acids and proteins and the techniques employed for successful determinations.
Original language | English (US) |
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Title of host publication | Analytical Techniques in Biosciences |
Subtitle of host publication | from Basics to Applications |
Publisher | Elsevier |
Pages | 147-156 |
Number of pages | 10 |
ISBN (Electronic) | 9780128226544 |
ISBN (Print) | 9780128227992 |
DOIs | |
State | Published - Jan 1 2022 |
Externally published | Yes |
Keywords
- chromatography
- DNA
- Protein
- RNA molecular weight
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)